caa a22 4500 606177442 CHVBK 20210128100752.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00018-015-1868-4 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1868-4 Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function [Elektronische Daten] [Sadat Dokanehiifard, Bahram Soltani, Sepideh Parsi, Fahimeh Hosseini, Mohammad Javan, Seyed Mowla] Tropomyosin receptor kinase C (TrkC) is involved in cell survival, apoptosis induction and tumorigenesis. We hypothesized that, similar to p75NTR receptor, some of the diverse functions of TrkC could be mediated by a microRNA (miRNA) embedded within the gene. Here, we experimentally verified the expression and processing of two bioinformatically predicted miRNAs named TrkC-miR1-5p and TrkC-miR1-3p. Transfecting a DNA fragment corresponding to the TrkC-premir1 sequence in HEK293t cells caused ~300-fold elevation in the level of mature TrkC-miR1 and also a significant downregulation of its predicted target genes. Furthermore, endogenous TrkC-miR1 was detected in several cell lines and brain tumors confirming its endogenous generation. Furthermore, its orthologous miRNA was detected in developing rat brain. Accordingly, TrkC-miR1 expression was increased during the course of neural differentiation of NT2 cell, whereas its suppression attenuated NT2 differentiation. Consistent with opposite functions of TrkC, TrkC-miR1 overexpression promoted survival and apoptosis in U87 and HEK293t cell lines, respectively. In conclusion, our data report the discovery of a new miRNA with overlapping function to TrkC. Springer Basel, 2015 TrkC - miR1 nationallicence Survival nationallicence U87 nationallicence Neural differentiation nationallicence Neurotrophin receptor nationallicence Dokanehiifard Sadat Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut Soltani Bahram Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut Parsi Sepideh Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut Hosseini Fahimeh Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut Javan Mohammad Department of Physiology, School of Medicine, Tarbiat Modares University, Tehran, Iran aut Mowla Seyed Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut Cellular and Molecular Life Sciences Springer Basel 72/13(2015-07-01), 2613-2625 1420-682X 72:13<2613 2015 72 18 https://doi.org/10.1007/s00018-015-1868-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1868-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dokanehiifard Sadat Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Soltani Bahram Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Parsi Sepideh Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Hosseini Fahimeh Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Javan Mohammad Department of Physiology, School of Medicine, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Mowla Seyed Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/13(2015-07-01), 2613-2625 1420-682X 72:13<2613 2015 72 18