caa a22 4500 606177493 CHVBK 20210128100752.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00018-014-1809-7 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1809-7 Circadian clocks in rat skin and dermal fibroblasts: differential effects of aging, temperature and melatonin [Elektronische Daten] [Cristina Sandu, Taole Liu, André Malan, Etienne Challet, Paul Pévet, Marie-Paule Felder-Schmittbuhl] As a peripheral tissue localized at the interface between internal and external environments, skin performs functions which are critical for the preservation of body homeostasis, in coordination with environmental changes. Some of these functions undergo daily variations, such as temperature or water loss, suggesting the presence of time-keeping mechanisms. Rhythmic functions are controlled by a network of circadian oscillators present virtually in every cell and coordinated by the central clock located in the suprachiasmatic nuclei. At the molecular level, circadian rhythms are generated by conserved transcriptional-translational feedback loops involving several clock genes, among which Per1 and Per2 play a central role. Here we characterize clock activity in skin of the transgenic Per1-luciferase rat during postnatal development and adulthood, by real-time recording of bioluminescence in explants and primary dermal fibroblasts, and report marked transformation in circadian properties, from early life to aging. Using primary dermal fibroblast cultures we provide evidence that melatonin treatment phase dependently increases the amplitude of circadian oscillations and that ambient temperature impacts on their period, with slight overcompensation. Together, these findings demonstrate that skin contains a self-sustained circadian clock undergoing age-dependent changes. Dermal fibroblasts, one of the major skin cell types, also exhibit robust, yet specific, circadian rhythmicity which can be fine-tuned by both internal (melatonin) and external (temperature) factors. Springer Basel, 2015 Per1 nationallicence Circadian rhythms nationallicence Development nationallicence Age nationallicence Bioluminescence nationallicence Sandu Cristina Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Liu Taole Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Malan André Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Challet Etienne Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Pévet Paul Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Felder-Schmittbuhl Marie-Paule Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut Cellular and Molecular Life Sciences Springer Basel 72/11(2015-06-01), 2237-2248 1420-682X 72:11<2237 2015 72 18 https://doi.org/10.1007/s00018-014-1809-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1809-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sandu Cristina Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 700 1- Liu Taole Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 700 1- Malan André Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 700 1- Challet Etienne Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 700 1- Pévet Paul Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 700 1- Felder-Schmittbuhl Marie-Paule Department of Neurobiology of Rhythms, Institute of Cellular and Integrative Neurosciences, UPR 3212 CNRS, Université de Strasbourg, 5 rue Blaise Pascal, 67084, Strasbourg, France aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/11(2015-06-01), 2237-2248 1420-682X 72:11<2237 2015 72 18