caa a22 4500 606177582 CHVBK 20210128100752.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00018-015-1968-1 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1968-1 Regulation of pannexin and connexin channels and their functional role in skeletal muscles [Elektronische Daten] [Juan Sáez, Bruno Cisterna, Anibal Vargas, Christopher Cardozo] Myogenic precursor cells express connexins (Cx) and pannexins (Panx), proteins that form different membrane channels involved in cell-cell communication. Cx channels connect either the cytoplasm of adjacent cells, called gap junction channels (GJC), or link the cytoplasm with the extracellular space, termed hemichannels (HC), while Panx channels only support the latter. In myoblasts, Panx1 HCs play a critical role in myogenic differentiation, and Cx GJCs and possibly Cx HCs coordinate metabolic responses during later steps of myogenesis. After innervation, myofibers do not express Cxs, but still express Panx1. In myotubes and innervated myofibers, Panx1 HCs allow release of adenosine triphosphate and thus they might be involved in skeletal muscle plasticity. In addition, Panx1 HCs present in adult myofibers mediate adenosine triphosphate release and glucose uptake required for potentiation of muscle contraction. Under pathological conditions, such as upon denervation and spinal cord injury, levels of Panx1 are upregulated. However, Panx1−/− mice show similar degree of atrophy as denervated wild-type muscles. Skeletal muscles also express Cx HCs in the sarcolemma after denervation or spinal cord injury, plus other non-selective membrane channels, including purinergic P2X7 receptors and transient receptor potential type V2 channels. The absence of Cx43 and Cx45 is sufficient to drastically reduce denervation atrophy. Moreover, inflammatory cytokines also induce the expression of Cxs in myofibers, suggesting the expression of these Cxs as a common factor for myofiber degeneration under diverse pathological conditions. Inhibitors of skeletal muscle Cx HCs could be promising tools to prevent muscle wasting induced by conditions associated with synaptic dysfunction and inflammation. Springer Basel, 2015 Pannexons nationallicence Connexons nationallicence Myoblasts nationallicence Myotubes nationallicence Striate muscles nationallicence Myogenesis nationallicence Denervation nationallicence ADP : Adenosine diphosphate nationallicence ATP : Adenosine triphosphate nationallicence Cx : Connexin nationallicence GJC : Gap junction channel nationallicence HC : Hemichannel nationallicence P2R : Purinergic P2 receptor nationallicence Panx : Pannexin nationallicence Sáez Juan Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut Cisterna Bruno Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut Vargas Anibal Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut Cardozo Christopher Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peters Veterans Affairs Medical Center, 10468, Bronx, NY, USA aut Cellular and Molecular Life Sciences Springer Basel 72/15(2015-08-01), 2929-2935 1420-682X 72:15<2929 2015 72 18 https://doi.org/10.1007/s00018-015-1968-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1968-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sáez Juan Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut NATIONALLICENCE 700 1- Cisterna Bruno Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut NATIONALLICENCE 700 1- Vargas Anibal Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile aut NATIONALLICENCE 700 1- Cardozo Christopher Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peters Veterans Affairs Medical Center, 10468, Bronx, NY, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/15(2015-08-01), 2929-2935 1420-682X 72:15<2929 2015 72 18