caa a22 4500 606181415 CHVBK 20210128100810.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10876-014-0744-y doi (NATIONALLICENCE)springer-10.1007/s10876-014-0744-y Cytotoxicity and Genotoxicity of Biosynthesized Gold and Silver Nanoparticles on Human Cancer Cell Lines [Elektronische Daten] [Asra Parveen, Srinath Rao] Nanoparticles research is currently an area of passionate scientific interest due to its wide variety of potential applications in therapeutic and biomedical interest. This paper presents cytotoxicity and genotoxicity of gold and silver nanoparticles synthesized by using Cassia auriculata leaf extract at room temperature on different cancer cell lines. The characterization was performed by UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and Transmission Electron Measurement (TEM). Cytotoxicity was analyzed against human carcinoma cells lines by MTT assay, while genotoxicity was monitored by agarose gel electrophoresis method. The UV-Vis spectroscopy reveals surface plasmon absorption maxima at 541nm for gold and 425nm for silver. The peaks in XRD pattern were in good agreement with the standard values of the face centered cubic form, with an average size of 21nm in gold and 20nm in silver. TEM reveals that the particles were spherical and polydisperse. This biological procedure for synthesis of AuNPs and AgNPs and selective inhibition of cancerous cells opens an alternative avenue to treat human cancer effectively. Least concentration of AgNPs was more toxic and AuNPs reveals dose dependent response. Springer Science+Business Media New York, 2014 Nanoparticles nationallicence Biological synthesis nationallicence Characterization nationallicence Cytotoxicity nationallicence Genotoxicity nationallicence Parveen Asra Plant Tissue Culture and Genetic Engineering Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, 585106, Gulbarga, Karnataka, India aut Rao Srinath Plant Tissue Culture and Genetic Engineering Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, 585106, Gulbarga, Karnataka, India aut Journal of Cluster Science Springer US; http://www.springer-ny.com 26/3(2015-05-01), 775-788 1040-7278 26:3<775 2015 26 10876 https://doi.org/10.1007/s10876-014-0744-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10876-014-0744-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parveen Asra Plant Tissue Culture and Genetic Engineering Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, 585106, Gulbarga, Karnataka, India aut NATIONALLICENCE 700 1- Rao Srinath Plant Tissue Culture and Genetic Engineering Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, 585106, Gulbarga, Karnataka, India aut NATIONALLICENCE 773 0- Journal of Cluster Science Springer US; http://www.springer-ny.com 26/3(2015-05-01), 775-788 1040-7278 26:3<775 2015 26 10876