caa a22 4500 606182039 CHVBK 20210128100814.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10876-015-0869-7 doi (NATIONALLICENCE)springer-10.1007/s10876-015-0869-7 Bacteria Generated Antibacterial Gold Nanoparticles and Potential Mechanistic Insight [Elektronische Daten] [Satyapriya Jena, Bharat Das, Ranadhir Bosu, Mrutyunjay Suar, Dindyal Mandal] In this article, industrially useful bacteria Bacillus megaterium (MTCC 2444) have been explored in the biosynthesis of gold nanoparticles for the first time. Different parameters including pH, temperature, biomass concentration have been studied for optimization of nanoparticle synthesis. Characterizations of nanoparticles have been carried out by UV-Vis spectroscopy, XRD, FTIR and HRTEM. The average size of the nanoparticles is found to be around 16nm. Mechanistic studies indicate that a flavin-containing reductase is possibly involved in the biosynthesis process. To the best of our knowledge, this is for the first time the participation of flavin-containing reductase domain of cytochrome P450 BM3 of B.megaterium is demonstrated in the biosynthesis of nanoparticles. Extracellular biosynthesis of gold nanoparticles has also been demonstrated by using the cell-free-extract of bacterial biomass. Moreover, biosynthesized gold nanoparticles exhibit wide spectrum of antimicrobial activities against several multidrug resistant pathogenic bacteria. Springer Science+Business Media New York, 2015 Gold nanoparticles nationallicence Biosynthesis nationallicence Bacillus megaterium nationallicence Reductase nationallicence Antimicrobial nationallicence Jena Satyapriya School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut Das Bharat School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut Bosu Ranadhir Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, India aut Suar Mrutyunjay School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut Mandal Dindyal School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut Journal of Cluster Science Springer US; http://www.springer-ny.com 26/5(2015-09-01), 1707-1721 1040-7278 26:5<1707 2015 26 10876 https://doi.org/10.1007/s10876-015-0869-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10876-015-0869-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jena Satyapriya School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut NATIONALLICENCE 700 1- Das Bharat School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut NATIONALLICENCE 700 1- Bosu Ranadhir Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, India aut NATIONALLICENCE 700 1- Suar Mrutyunjay School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut NATIONALLICENCE 700 1- Mandal Dindyal School of Biotechnology, KIIT University, Campus 11, Patia, 751024, Bhubaneswar, Odisha, India aut NATIONALLICENCE 773 0- Journal of Cluster Science Springer US; http://www.springer-ny.com 26/5(2015-09-01), 1707-1721 1040-7278 26:5<1707 2015 26 10876