caa a22 4500 606182438 CHVBK 20210128100815.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00405-014-3226-9 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3226-9 Expression of atrophy-related transcription factors in the process of intrinsic laryngeal muscle atrophy after denervation [Elektronische Daten] [Hirofumi Sei, Aki Taguchi, Naoya Nishida, Naohito Hato, Kiyofumi Gyo] We examined changes in the expressions of three atrophy-related transcription factors (FOXO3a, P-FOXO3a, and PGC-1α) in the process of intrinsic laryngeal muscle atrophy after denervation. In total, 51 Wistar rats were used. After transection of the unilateral recurrent laryngeal nerve, the thyroarytenoid (TA) muscle and the posterior cricoarytenoid (PCA) muscle were excised and subjected to histological and Western blot studies. Relationships between the expressions of transcription factors during atrophy of the intrinsic laryngeal muscles were investigated by comparing the results of the treated side (T) with those of the untreated side (U), and sequential changes in the T/U ratio after denervation were assessed. Loss of wet muscle weight, together with a decrease in muscle fiber cross-sectional area and increase in the number of muscle fibers/mm2, occurred more quickly in TA muscle than in PCA muscle. Muscle atrophy progressed rapidly between 7 and 28days after denervation, while expression of FOXO3a was maximal on day 7, in both TA and PCA muscles. By contrast, P-FOXO3a expression decreased gradually after denervation. Expression of PGC-1α increased slowly until day 7, and then it declined. Denervation-induced atrophy of the intrinsic laryngeal muscles was closely linked with the expression of FOXO3a and PGC-1α, suggesting that atrophy of these muscles may involve the actions of these transcription factors. In addition, muscle atrophy progressed faster in TA muscle than in PCA muscle, due mainly to differences in muscle fiber composition. Springer-Verlag Berlin Heidelberg, 2014 Laryngeal muscle atrophy nationallicence Intrinsic laryngeal muscle nationallicence FOXO3a nationallicence Phosphorylated FOXO3a nationallicence PGC-1α nationallicence Sei Hirofumi Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut Taguchi Aki Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut Nishida Naoya Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut Hato Naohito Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut Gyo Kiyofumi Department of Otolaryngology, Takanoko Hospital, Takanoko Town 525-1, 790-0925, Matsuyama, Ehime, Japan aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/1(2015-01-01), 137-141 0937-4477 272:1<137 2015 272 405 https://doi.org/10.1007/s00405-014-3226-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3226-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sei Hirofumi Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut NATIONALLICENCE 700 1- Taguchi Aki Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut NATIONALLICENCE 700 1- Nishida Naoya Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut NATIONALLICENCE 700 1- Hato Naohito Department of Otolaryngology, Ehime University School of Medicine, Shitsukawa, 91-0295, Toon, Ehime, Japan aut NATIONALLICENCE 700 1- Gyo Kiyofumi Department of Otolaryngology, Takanoko Hospital, Takanoko Town 525-1, 790-0925, Matsuyama, Ehime, Japan aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/1(2015-01-01), 137-141 0937-4477 272:1<137 2015 272 405