caa a22 4500 606182462 CHVBK 20210128100816.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00405-013-2832-2 doi (NATIONALLICENCE)springer-10.1007/s00405-013-2832-2 Effect of caffeic acid phenethyl ester on myringosclerosis development in the tympanic membrane of rat [Elektronische Daten] [Vefa Kinis, Musa Ozbay, Ulas Alabalik, Aylin Gul, Beyhan Yilmaz, Fazi Ozkurt, Engin Sengul, Ismail Topcu] Myringosclerosis is hyalinization and calcification of certain areas of the tympanic membrane, especially the fibrous lamina propria layer and appears as white sclerotic lesions. Ventilation tube insertion is one of the most performed operations in the pediatric otorhinolaryngology practice to treat chronic otitis media with effusion. Myringosclerosis is a very common sequela of ventilation tube insertion. In this experimental study, our aim was to show the histopathological effects of caffeic acid phenethyl ester on myringosclerosis development in rat tympanic membrane after myringotomy. The rats were randomly categorized into four experimental groups including the comparison group (n=4), non-treated group (n=7), the saline (control) group (n=7), the caffeic acid phenethyl ester group (n=7). Non-treated group did not receive any treatment for 15days. Saline (2.5mL/kg, intraperitoneal) was administered to the third group once a day for 15days. Fourth group received caffeic acid phenethyl ester intraperitoneally once a day at a dose of 10μmol/kg for 15days. Myringotomy was performed on the right tympanic membrane of all rats except comparison group using a sterile pick with the help of an operating microscope. Histopathological examination of myringosclerosis formation was done by a pathologist under light microscope. In histopathological analysis of groups, the severity of inflammation was milder in caffeic acid phenethyl ester group compared to non-treated and saline groups (p<0.05). There was less myringosclerotic plaques in caffeic acid phenethyl ester group than in non-treated and saline groups (p<0.05). TM thickness measurements were very close to each other in non-treated and saline groups. The tympanic membrane thickness of caffeic acid phenethyl ester group was much thinner than the other two groups (p<0.05). Caffeic acid phenethyl ester decreases inflammation severity and the formation of myringosclerotic plaques. These two effects resulted in thinner tympanic membranes of rats which were treated with caffeic acid phenethyl ester. As a result, caffeic acid phenethyl ester has potential preventive effects on myringosclerosis development after myringotomy and ventilation tube insertion. Springer-Verlag Berlin Heidelberg, 2013 Caffeic acid phenethyl ester nationallicence Myringosclerosis nationallicence Ventilation tube nationallicence Tympanic membrane nationallicence Inflammation nationallicence Rat nationallicence Kinis Vefa Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Ozbay Musa Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Alabalik Ulas Department of Pathology, Dicle University Medical College, Diyarbakir, Turkey aut Gul Aylin Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Yilmaz Beyhan Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Ozkurt Fazi Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Sengul Engin Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut Topcu Ismail Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/1(2015-01-01), 29-34 0937-4477 272:1<29 2015 272 405 https://doi.org/10.1007/s00405-013-2832-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-013-2832-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kinis Vefa Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Ozbay Musa Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Alabalik Ulas Department of Pathology, Dicle University Medical College, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Gul Aylin Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Yilmaz Beyhan Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Ozkurt Fazi Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Sengul Engin Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 700 1- Topcu Ismail Department of ENT & Head and Neck Surgery, Dicle University Medical College, 21280, Diyarbakir, Turkey aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/1(2015-01-01), 29-34 0937-4477 272:1<29 2015 272 405