caa a22 4500 606183450 CHVBK 20210128100821.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00405-014-3093-4 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3093-4 Radix astragali inhibits the down-regulation of connexin 26 in the stria vascularis of the guinea pig cochlea after acoustic trauma [Elektronische Daten] [Min Xiong, Yazhen Zhu, Huangwen Lai, Xiaoyan Fu, Wenting Deng, Chuanhong Yang, Qinglian He, Guangjuan Zheng] Connexin 26 (cx26) plays an important role in the intercellular signaling and is related to K+ metabolism in stria vascularis (SV). Reactive oxygen species (ROS) are negative regulators of cx26, reducing intercellular coupling in cochlea. ROS plays an important role in acoustic trauma. Radix astragali is a natural antioxidant that decreases impulse noise-induced hearing loss through its ability to inhibit ROS. The purpose of this study was to investigate if radix astragali has the potential to reduce the change of cx26 in SV from impulse noise. Guinea pigs in the experimental group were administered radix astragali intraperitoneally. Auditory thresholds were assessed by sound-evoked auditory brainstem response (ABR) at click and tone bursts of 8, 16 and 32kHz, 24h before and 72h after exposure to impulse noise. 4-Hydroxynonenal, cx26 and KCNQ1 were determined immunohistochemically in SV. SV was analyzed by transmission electron microscopy. Radix astragali significantly reduced the ABR deficits and the SV damage, and decreased the shifts of the expression of cx26 and KCNQ1 in the SV. These results suggest that the beneficial effect of radix astragali on impulse noise-induced hearing loss may be also due to its ability to reduce the change of cx26 in SV. Springer-Verlag Berlin Heidelberg, 2014 Impulse noise nationallicence Cochlea nationallicence Hearing loss nationallicence Radix astragali nationallicence Connexin 26 nationallicence Reactive oxygen species nationallicence Xiong Min Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut Zhu Yazhen Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut Lai Huangwen Medical Research Center, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, China aut Fu Xiaoyan Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut Deng Wenting Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut Yang Chuanhong Medical Research Center, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, China aut He Qinglian Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut Zheng Guangjuan Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/9(2015-09-01), 2153-2160 0937-4477 272:9<2153 2015 272 405 https://doi.org/10.1007/s00405-014-3093-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3093-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiong Min Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut NATIONALLICENCE 700 1- Zhu Yazhen Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Lai Huangwen Medical Research Center, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, China aut NATIONALLICENCE 700 1- Fu Xiaoyan Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut NATIONALLICENCE 700 1- Deng Wenting Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, 510010, Guangzhou, China aut NATIONALLICENCE 700 1- Yang Chuanhong Medical Research Center, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, China aut NATIONALLICENCE 700 1- He Qinglian Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut NATIONALLICENCE 700 1- Zheng Guangjuan Department of Pathology, The Second Hospital Affiliated To Guangzhou Traditional Chinese Medical University, Da De Road 111, 510120, Guangzhou, China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/9(2015-09-01), 2153-2160 0937-4477 272:9<2153 2015 272 405