caa a22 4500 606183795 CHVBK 20210128100823.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00405-014-3322-x doi (NATIONALLICENCE)springer-10.1007/s00405-014-3322-x Sinus biofilms in patients with cystic fibrosis: is adjusted eradication therapy needed? [Elektronische Daten] [Kasper Aanaes, Steffen Eickhardt, Helle Johansen, Christian von Buchwald, Marianne Skov, Niels Høiby, Thomas Bjarnsholt] The paranasal sinuses can be a focus for colonisation of the cystic fibrosis (CF) lungs with pathogens. In the sinuses, bacteria can adapt to the lung environment and enhance their antibiotic resistance, with biofilm formation thought to be the most important adaptive mechanism, causing recalcitrant disease. The presence of biofilms in CF sinuses is sparsely described. In this descriptive cross-sectional study, the sinus mucosa from 16 CF patients were analysed by fluorescence in situ hybridization using specific peptide nucleic acid (PNA-FISH) probes for Pseudomonas aeruginosa and Staphylococcus aureus to demonstrate the presence of biofilms. Small clusters of biofilm were visualised lining the sinus mucosa of CF patients. Biofilms were found in 10 out of 18 cases; 7 with intermittent lung colonisation, 2 chronically infected, and one lung transplanted patient. Finding P. aeruginosa biofilms in intermittently lung-colonised patients encourage us to intensify the attempt to eradicate pathogenic bacteria from the CF sinuses in an early stage using combined antibiotic therapy in the prolonged exposure of the sinus-mucosal surface. Springer-Verlag Berlin Heidelberg, 2014 Biofilm nationallicence Paranasal sinuses nationallicence Cystic fibrosis nationallicence Pseudomonas aeruginosa nationallicence Staphylococcus aureus nationallicence Aanaes Kasper Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Rigshospitalet and Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark aut Eickhardt Steffen Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut Johansen Helle Department of Clinical Microbiology 9301, Rigshospitalet, Juliane Maries Vej 22, 2100, Copenhagen, Denmark aut von Buchwald Christian Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Rigshospitalet and Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark aut Skov Marianne Paediatric Pulmonary Service Department of Paediatrics and Adolescent Medicine, Danish PCD and Child Centre, CF Centre Copenhagen, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark aut Høiby Niels Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut Bjarnsholt Thomas Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/9(2015-09-01), 2291-2297 0937-4477 272:9<2291 2015 272 405 https://doi.org/10.1007/s00405-014-3322-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3322-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aanaes Kasper Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Rigshospitalet and Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Eickhardt Steffen Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Johansen Helle Department of Clinical Microbiology 9301, Rigshospitalet, Juliane Maries Vej 22, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- von Buchwald Christian Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Rigshospitalet and Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Skov Marianne Paediatric Pulmonary Service Department of Paediatrics and Adolescent Medicine, Danish PCD and Child Centre, CF Centre Copenhagen, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Høiby Niels Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Bjarnsholt Thomas Institute of International Health, Immunology and Microbiology, The Faculty of Health Sciences, Panum Institute, The University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/9(2015-09-01), 2291-2297 0937-4477 272:9<2291 2015 272 405