caa a22 4500 60618435X CHVBK 20210128100826.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00405-015-3511-2 doi (NATIONALLICENCE)springer-10.1007/s00405-015-3511-2 Chronic rhinosinusitis: association of recalcitrant nasal polyposis and fungal finding in polyp's single-cell suspension [Elektronische Daten] [Aleksandra Barac, Marina Pekmezovic, Vesna Spiric, Aleksandar Trivic, Jelena Marinkovic, Sandra Pekic, Valentina Arsenijevic] In recent years fungi are favoured as origin of chronic rhinosinusitis (CRS), especially with nasal polyps (wNP). Sensitive methods for fungal detection are still absent, therefore we used NP tissue single-cell suspension for mycology investigations in patients with recalcitrant NP (rNP) that underwent functional endoscopic sinus surgery (FESS). A prospective case-series study and culture-based mycological examination were conducted in patients who underwent FESS for the first time (ft-FESS) and those with repeated FESS (re-FESS). The study was conducted in a tertiary Otorhinolaryngology Unit of Clinical Centre of Serbia. A total of 43 consecutive patients with CRSwNP underwent FESS. Culture-based mycological examination of single-cell suspension was done on 55 NPs samples. Patient's co-morbidity data were collected. Repeated FESS was observed in 19/43 (44%) patients (re-FESS group). Asthma and aspirin intolerance were more frequent in re-FESS than in ft-FESS group (p=0.000, p=0.002; respectively). Fungi were detected (wF) in 10/43 (23.3%) patients (FESSwF group), representing 13/55 culture positive NP tissue (23.6%). Fungal presence was higher in re-FESS than in ft-FESS group (42 and 8%, respectively; p=0.01). Significantlylonger duration of CRS was observed in FESSwF than in fungal negative patients (p=0.033). Predominate strain was Aspergillus flavus detected in 6/10 patients. This is the first study which analysed association of fungi in single-cell suspension of NP tissue and rNP. We demonstrate significantly higher percentage of positive fungal finding in re-FESSwF than in ft-FESSwF group. The most commonly isolated species in our patients was A. flavus. Springer-Verlag Berlin Heidelberg, 2015 Chronic rhinosinusitis nationallicence Recalcitrant nasal polyposis nationallicence Functional endoscopic sinus surgery nationallicence Fungi nationallicence Tissue nationallicence Single-cell suspension nationallicence Barac Aleksandra Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut Pekmezovic Marina Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut Spiric Vesna Clinic for Allergology and Immunology, Clinical Centre of Serbia, Belgrade, Serbia aut Trivic Aleksandar Faculty of Medicine, University of Belgrade, Belgrade, Serbia aut Marinkovic Jelena Faculty of Medicine, Institute of Medical Statistics and Informatics, University of Belgrade, Belgrade, Serbia aut Pekic Sandra Faculty of Medicine, University of Belgrade, Belgrade, Serbia aut Arsenijevic Valentina Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/12(2015-12-01), 3727-3734 0937-4477 272:12<3727 2015 272 405 https://doi.org/10.1007/s00405-015-3511-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-015-3511-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barac Aleksandra Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut NATIONALLICENCE 700 1- Pekmezovic Marina Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut NATIONALLICENCE 700 1- Spiric Vesna Clinic for Allergology and Immunology, Clinical Centre of Serbia, Belgrade, Serbia aut NATIONALLICENCE 700 1- Trivic Aleksandar Faculty of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Marinkovic Jelena Faculty of Medicine, Institute of Medical Statistics and Informatics, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Pekic Sandra Faculty of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Arsenijevic Valentina Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Dr Subotica 1, Belgrade, Serbia aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/12(2015-12-01), 3727-3734 0937-4477 272:12<3727 2015 272 405