caa a22 4500 606184503 CHVBK 20210128100826.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00405-014-3481-9 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3481-9 Significance of CYCLOOXYGENASE - 2(COX - 2), PERIOSTIN (POSTN) and INTERLEUKIN - 4(IL - 4) gene expression in the pathogenesis of chronic rhinosinusitis with nasal polyps [Elektronische Daten] [Jarosław Miłoński, Hanna Zielińska-Bliźniewska, Karolina Przybyłowska, Piotr Pietkiewicz, Barbara Korzycka-Zaborowska, Ireneusz Majsterek, Jurek Olszewski] The purpose of this paper was to evaluate the level of Cyclooxygenase-2 (COX-2), Periostin (POSTN) and Interleukin-4(IL-4) gene expression in patients with chronic rhinosinusitis with nasal polyps, without polyps and with a nasal septum deviation. The tests were performed on 63 patients (24 women and 39 men) with chronic rhinosinusitis and polyps (CRSwP—study group I), with determination of the COX-2, POSTN and IL-4 gene expression; an allergy was diagnosed in 38 cases. The reference groups were patients with chronic rhinosinusitis without polyps--CRS (n=23, including 14 women and 9 men) and patients with nasal septum deviation--DSN (n=18, including 9 women and 9 men). The expression level was determined in the polyp tissue and the mucosa of paranasal sinus collected during an FESS. The expression level of studied genes was also evaluated in the material. Immediately after being collected, the tissue fragments were placed in test tubes with 1ml of RNAlater (Qiagen, Hilden, Germany) preventing the degradation of RNA and frozen at −70°C. The studies revealed an increased level of POSTN, IL-4 gene expression and a decreased level of COX-2 gene expression that may be associated with the development of chronic rhinosinusitis with nasal polyps. An analysis of the expression level indicates the participation of POSTN and IL-4 in the development of chronic rhinosinusitis with nasal polyps in patients with atopy. The Author(s), 2015 Gene expression nationallicence Chronic rhinosinusitis with nasal polyps nationallicence Miłoński Jarosław Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut Zielińska-Bliźniewska Hanna Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut Przybyłowska Karolina Department of Chemistry and Clinical Biochemistry, Medical University of Lodz, Lodz, Poland aut Pietkiewicz Piotr Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut Korzycka-Zaborowska Barbara Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut Majsterek Ireneusz Department of Chemistry and Clinical Biochemistry, Medical University of Lodz, Lodz, Poland aut Olszewski Jurek Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/12(2015-12-01), 3715-3720 0937-4477 272:12<3715 2015 272 405 https://doi.org/10.1007/s00405-014-3481-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3481-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miłoński Jarosław Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut NATIONALLICENCE 700 1- Zielińska-Bliźniewska Hanna Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut NATIONALLICENCE 700 1- Przybyłowska Karolina Department of Chemistry and Clinical Biochemistry, Medical University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Pietkiewicz Piotr Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut NATIONALLICENCE 700 1- Korzycka-Zaborowska Barbara Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut NATIONALLICENCE 700 1- Majsterek Ireneusz Department of Chemistry and Clinical Biochemistry, Medical University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Olszewski Jurek Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, Zeromskiego 113, 90-549, Lodz, Poland aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/12(2015-12-01), 3715-3720 0937-4477 272:12<3715 2015 272 405