caa a22 4500 606184686 CHVBK 20210128100827.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00405-013-2842-0 doi (NATIONALLICENCE)springer-10.1007/s00405-013-2842-0 Safety of transtympanic application of 4% manuka honey in a chinchilla animal model [Elektronische Daten] [M. Aron, O. Akinpelu, K. Gasbarrino, S. Daniel] The antimicrobial and anti-biofilm properties of manuka honey (MH) are currently being explored in the treatment of chronic recalcitrant rhinosinusitis. Due to similarities between chronic rhinosinusitis and chronic otitis, manuka honey may find applications in the management of challenging cases of chronic otitis media implicating biofilms. The goal of this study was to investigate the safety of topical application of 4% MH in the middle ear. Eleven adult female chinchillas had one of their ears randomly assigned to receive transtympanic 4% MH, while the contralateral ear served as control. Auditory brainstem-evoked response (ABR) was performed before and after MH application. The facial nerve function and vestibular system were assessed clinically. The animals were euthanized one month following the last application, and the cochleae samples were processed for light and scanning electron microscopy. There was no statistically significant differences between ABR thresholds in both control and experimental ears before and after the application of MH. No morphological differences were seen in both groups of cochleae. The outer hair cell counts for both groups were comparable. Our results suggest that 4% MH appears not toxic to the cells of the cochlea after 4weeks of application. The long-term effects of prolonged contact on the structure and function of the cochlea however needfurther investigations. Springer-Verlag Berlin Heidelberg, 2013 Manuka honey nationallicence 4% nationallicence Ototoxicity nationallicence Transtympanic nationallicence ABR nationallicence Cochlear histology light microscopy nationallicence Scanning electron microscopy nationallicence Aron M. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut Akinpelu O. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut Gasbarrino K. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut Daniel S. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 537-542 0937-4477 272:3<537 2015 272 405 https://doi.org/10.1007/s00405-013-2842-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-013-2842-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aron M. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut NATIONALLICENCE 700 1- Akinpelu O. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut NATIONALLICENCE 700 1- Gasbarrino K. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut NATIONALLICENCE 700 1- Daniel S. Department of Otolaryngology, Head and Neck Surgery, McGill University, Montreal, Canada aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 537-542 0937-4477 272:3<537 2015 272 405