caa a22 4500 606184716 CHVBK 20210128100828.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00405-014-3146-8 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3146-8 Downregulation of caveolin-1 in chronic rhinosinusitis with and without nasal polyps [Elektronische Daten] [Hai Lin, Dong Lin, Xi-Sheng Xiong, Xiong-Xiong Dai, Ting Lin] The pathogenesis of human chronic rhinosinusitis (CRS) remains controversial. Recent evidence has suggested that caveolin-1 (Cav-1) is a 22kDa scaffolding protein and plays a pivotal role in host defense against infections and tumour suppression by reducing production of cyclin D1 and endothelial nitric oxide-synthase (eNOS). However, little is known about their roles in CRS. Therefore, we aimed to investigate the expression and role of Cav-1 in CRS. Cav-1 protein expression were investigated by immunohistochemistry method and mRNA expression of Cav-1, cyclin D1 and eNOS were assessed by real-time polymerase chain reaction in CRS and control subjects. Moreover, the effects of various stimulators with different concentrations and time on Cav-1 were evaluated on nasal explant culture. The results showed that weaker expression of Cav-1 protein and mRNA were observed in CRS, especially in CRS with nasal polyps (CRSwNP), stronger mRNA expression of cyclin D1 and eNOS were observed in CRS and Cav-1 expression was negatively related to cyclin D1 and eNOS expression, respectively. Cav-1 mRNA was augmented by IFN-γ, but supressed by IL-4 and IL-1β. In conclusion, the expression of Cav-1 was downregulated in CRS and the role of Cav-1 was impaired in CRS, especially in CRSwNP, leading to the attenuation of inhibition effect on cyclin D1 and eNOS and resulted in the overexpression of cyclin D1 and eNOS. IFN-γ may be essential for Cav-1 gene expression. Springer-Verlag Berlin Heidelberg, 2014 Caveolin-1 nationallicence Caveolae nationallicence Cyclin D1 nationallicence Endothelial nitric oxide-synthase nationallicence Chronic rhinosinusitis nationallicence Nasal polyps nationallicence Lin Hai Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Xuhui District, 200031, Shanghai, China aut Lin Dong Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut Xiong Xi-Sheng Department of Pathology, Fuzhou General Hospital, 350025, Fuzhou, Fujian, China aut Dai Xiong-Xiong Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut Lin Ting Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 613-618 0937-4477 272:3<613 2015 272 405 https://doi.org/10.1007/s00405-014-3146-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3146-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Hai Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Xuhui District, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Lin Dong Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut NATIONALLICENCE 700 1- Xiong Xi-Sheng Department of Pathology, Fuzhou General Hospital, 350025, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Dai Xiong-Xiong Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut NATIONALLICENCE 700 1- Lin Ting Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, 350300, Fuqing, Fujian, China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 613-618 0937-4477 272:3<613 2015 272 405