caa a22 4500 606184740 CHVBK 20210128100828.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00405-013-2864-7 doi (NATIONALLICENCE)springer-10.1007/s00405-013-2864-7 Bacterial change in external auditory canal upon antisepsis with povidone-iodine during tympanoplasty [Elektronische Daten] [Ilker Arslan, Selahattin Genc, Bekir Kayhan, Murat Gumussoy, Gonul Ozel, Ibrahim Cukurova] The aim of this single-arm prospective study was to determine the flora of the external auditory canal (EAC) in inactive chronic otitis media and evaluate the alteration of microorganisms of the EAC during tympanoplasty upon povidone-iodine antisepsis. Sixty-three patients with central tympanic membrane perforation were enrolled in the study. Preoperative swab cultures were obtained and the EAC was packed with povidone-iodine absorbed gauze. Type I tympanoplasty via a retroauricular route was performed. Cultures from the EAC were taken at the end of each operation. Isolated organisms were identified based upon microbiological, morphological, and biochemical characteristics. The most commonly isolated organisms from preoperative samples were normal commensal flora, including 73 coagulase-negative staphylococci (CNS) and 18 diphtheroid bacilli (DB). Less commonly cultured pathogenic species included four isolates of Staphylococcus aureus and three isolates of Candida albicans. No bacteria were observed in five patients. Following povidone-iodine antisepsis, 32 of the samples were negative. Eradication was statistically significant for CNS, DB and pathogen microorganism (P<0.05). Isolated bacteria differed from those in preoperative swab cultures in eight cases. After antisepsis, diverse strains of the CNS were isolated in 13 cases and 10 patients showed no change in microbial flora. Postoperative culture demonstrated that all seven pathogenic isolates were eradicated (100%); this selective efficacy of povidone-iodine antisepsis against pathogenic isolates was significant when compared with commensal flora (P<0.05). These results suggest that povidone-iodine antisepsis of the EAC before tympanoplasty is an effective method for the elimination microorganisms, especially pathogenic bacteria. Springer-Verlag Berlin Heidelberg, 2013 Flora of external auditory canal nationallicence Inactive chronic otitis media nationallicence Povidone-iodine nationallicence Antisepsis nationallicence Tympanoplasty nationallicence Arslan Ilker Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut Genc Selahattin Derince Training and Research Hospital Head and Neck Surgery Clinic, Kocaeli, Turkey aut Kayhan Bekir Yozgat Government Hospital Infectious Diseases and Microbiology Clinic, Yozgat, Turkey aut Gumussoy Murat Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut Ozel Gonul Yozgat Government Hospital Infectious Diseases and Microbiology Clinic, Yozgat, Turkey aut Cukurova Ibrahim Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 551-555 0937-4477 272:3<551 2015 272 405 https://doi.org/10.1007/s00405-013-2864-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-013-2864-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arslan Ilker Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut NATIONALLICENCE 700 1- Genc Selahattin Derince Training and Research Hospital Head and Neck Surgery Clinic, Kocaeli, Turkey aut NATIONALLICENCE 700 1- Kayhan Bekir Yozgat Government Hospital Infectious Diseases and Microbiology Clinic, Yozgat, Turkey aut NATIONALLICENCE 700 1- Gumussoy Murat Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut NATIONALLICENCE 700 1- Ozel Gonul Yozgat Government Hospital Infectious Diseases and Microbiology Clinic, Yozgat, Turkey aut NATIONALLICENCE 700 1- Cukurova Ibrahim Tepecik Training and Research Hospital Head and Neck Surgery Clinic, Izmir, Turkey aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 551-555 0937-4477 272:3<551 2015 272 405