caa a22 4500 606184791 CHVBK 20210128100828.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00405-014-2984-8 doi (NATIONALLICENCE)springer-10.1007/s00405-014-2984-8 Photosensitizing effects of hypericin on head neck squamous cell carcinoma in vitro [Elektronische Daten] [Wiebke Laffers, Ann-Christin Busse, Jens Mahrt, Phuc Nguyen, Andreas Gerstner, Friedrich Bootz, Johannes Wessels] Clinical outcome of patients suffering from head neck squamous cell carcinomas is still poor due to recurrent disease and surgical limitations. There is still a demand for multimodality approaches and new therapeutic options. Hypericin is a promising phototoxic drug which was investigated for its effects on head neck squamous cell carcinoma cells in vitro. FaDu cells incubated with or without hypericin were illuminated (450-700nm, 50,000lx) for different time periods. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide- and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay were used to score metabolic and apoptotic activity. Even after the shortest illumination FaDu cells incubated with hypericin showed massive reduction of metabolism and excessive apoptosis. This was present even with the lowest hypericin concentration. Cells without hypericin or without illumination were not affected. These photosensitizing effects of hypericin could be suitable for clinical application and could lead to the development of an intraoperative photodynamic therapy of head neck squamous cell carcinomas. Springer-Verlag Berlin Heidelberg, 2014 Hypericin nationallicence FaDu nationallicence Head neck cancer nationallicence HNSCC nationallicence Photodynamic therapy (PDT) nationallicence Laffers Wiebke Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Busse Ann-Christin Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut Mahrt Jens Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut Nguyen Phuc Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut Gerstner Andreas Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Bootz Friedrich Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Wessels Johannes Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 711-718 0937-4477 272:3<711 2015 272 405 https://doi.org/10.1007/s00405-014-2984-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-2984-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laffers Wiebke Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Busse Ann-Christin Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Mahrt Jens Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Nguyen Phuc Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Gerstner Andreas Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Bootz Friedrich Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Wessels Johannes Department of Nephrology/Rheumatology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/3(2015-03-01), 711-718 0937-4477 272:3<711 2015 272 405