caa a22 4500 606184945 CHVBK 20210128100829.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-014-3284-z doi (NATIONALLICENCE)springer-10.1007/s00405-014-3284-z The lack of 4-hydroxynonenal in otosclerotic bone tissue in Ethiopian population [Elektronische Daten] [Milan Rudic, Richard Wagner, Eric Willkinson, Giovanni Danese, Nega Kiros, Kamelija Zarkovic, Neven Zarkovic] In Ethiopians, like in other Africans, the incidence of otosclerosis is lower than in Western and Asian populations. Unfortunately, due to the lack of available otorhinolaryngology specialists many patients are not treated and suffer the progression of the disease and severe hearing loss. This program of the Global ENT Outreach Organization (GEO) together with the Ethiopian partners was done to help some of these patients and in parallel to evaluate the presence of the oxidative stress bioactive marker 4-hydroxynonenal (HNE), which is known as major lipid peroxidation product and the second messenger of free radicals, in the otosclerotic bone specimens. Namely, we described recently that as HNE acts as a bone growth regulator associated with pathogenesis of otosclerosis. The prospective study conducted at the ENT Department of the Migbare Senay General Hospital, Addis Ababa, Ethiopia in June 2012, under the auspices of the Global ENT Outreach Organization, USA. Altogether 36 patients (male=12, female=24) underwent surgery due to the previous otosclerosis diagnosis based on the clinical and audiometric findings. The bone samples were harvested from patients with intraoperatively confirmed otosclerosis diagnosis. Immunohistochemistry for HNE-modified proteins was carried out on formalin-fixed paraffin-embedded specimens. The presence of HNE was found in almost all bone samples analyzed, without particular difference in the HNE distribution pattern between the otosclerotic and respective control bone specimens. Although there was no significant association between the HNE appearance and otosclerotic bone outgrowth observed, several cases have shown tendency of higher HNE expression in patients with more severe hearing loss. The results of the present study are in contrast with our previous findings obtained on European patients most likely due to the differences between studied population groups. Springer-Verlag Berlin Heidelberg, 2014 Oxidative stress nationallicence Otosclerosis nationallicence Pathophysiology nationallicence Rudic Milan ENT Department, General Hospital Zadar, B. Peričića 5, 23000, Zadar, Croatia aut Wagner Richard Global ENT Outreach Organization (GEO), Coupeville, USA aut Willkinson Eric Global ENT Outreach Organization (GEO), Coupeville, USA aut Danese Giovanni Global ENT Outreach Organization (GEO), Coupeville, USA aut Kiros Nega Migbare Senay General Hospital, Addis Ababa, Ethiopia aut Zarkovic Kamelija Division of Pathology, Clinical Hospital Centre Zagreb, University Medical School, Zagreb, Croatia aut Zarkovic Neven Institute Ruđer Bošković, Zagreb, Croatia aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2783-2789 0937-4477 272:10<2783 2015 272 405 https://doi.org/10.1007/s00405-014-3284-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3284-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rudic Milan ENT Department, General Hospital Zadar, B. Peričića 5, 23000, Zadar, Croatia aut NATIONALLICENCE 700 1- Wagner Richard Global ENT Outreach Organization (GEO), Coupeville, USA aut NATIONALLICENCE 700 1- Willkinson Eric Global ENT Outreach Organization (GEO), Coupeville, USA aut NATIONALLICENCE 700 1- Danese Giovanni Global ENT Outreach Organization (GEO), Coupeville, USA aut NATIONALLICENCE 700 1- Kiros Nega Migbare Senay General Hospital, Addis Ababa, Ethiopia aut NATIONALLICENCE 700 1- Zarkovic Kamelija Division of Pathology, Clinical Hospital Centre Zagreb, University Medical School, Zagreb, Croatia aut NATIONALLICENCE 700 1- Zarkovic Neven Institute Ruđer Bošković, Zagreb, Croatia aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2783-2789 0937-4477 272:10<2783 2015 272 405