caa a22 4500 606185135 CHVBK 20210128100830.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-014-3354-2 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3354-2 Effects of histamine on ciliary beat frequency of ciliated cells from guinea pigs nasal mucosa [Elektronische Daten] [Fengwei An, Lijun Xing, Zhiqiang Zhang, Lei Chen] We aimed to investigate the effect of histamine on ciliary beat frequency (CBF) through combining high-speed digital microscopy and patch-clamp technology. Ciliated cells were obtained from septum and turbinate of 90-120-day-old healthy male guinea pigs. Tight seal was formed by applying negative pressure on the glass electrode after the drawing and pushing progress. Then, we enrolled high-speed digital microscopy to measure CBF before and after treatment with histamine of different concentrations ranging from 10−6 to 10−1mol/L in Hank's solution and D-Hank's solution as well as after administrating adenosine triphosphate. One-way ANOVA, Student's t test or Kruskal-Wallis test was used for statistical comparisons. Glass electrode fix up ciliated cell is available at tip diameter of 2-5μm and negative pressure of 10-20cmH2O column. The baseline CBF in Hank's solution was higher than in D-Hank's solution. Treatment with 10−6-l0−3mol/L histamine of concentrations can stimulate a rise of CBF. Nevertheless, CBF in all groups decreased to baseline CBF within 20min. Generally, 10−2mol/L histamine can stimulate a rise of CBF; meanwhile, the high concentration of histamine killed 50% ciliated cell. Histamine at 10−1mol/L killed all ciliated cells. Ciliary beating activity decreased in Ca2+-free solution. Moreover, adenosine triphosphate could increase CBF effectively after the stimulation effect of histamine. We construct an effective technology integrating patch-clamp technique with CBF measurements on ciliated cells. Extracellular histamine stimulation could increase CBF effectively. Springer-Verlag Berlin Heidelberg, 2014 Patch-clamp nationallicence High-speed digital microscopy nationallicence CBF nationallicence Histamine nationallicence An Fengwei Department of Otorhinolaryngology, Jinan Military General Hospital, 250031, Jinan, Shandong Province, China aut Xing Lijun Department of Otorhinolaryngology, The People's Hospital of Rushan City, 264500, Weihai, Shandong Province, China aut Zhang Zhiqiang Department of Gastroenterology and Hepatology, People's Hospital of Huangdao, 266400, Qingdao, Shandong Province, China aut Chen Lei Department of Otorhinolaryngology, The General Hospital of People's Liberation Army, No.28, Fuxing Road, Haidian District, 100853, Beijing, China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2839-2845 0937-4477 272:10<2839 2015 272 405 https://doi.org/10.1007/s00405-014-3354-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3354-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- An Fengwei Department of Otorhinolaryngology, Jinan Military General Hospital, 250031, Jinan, Shandong Province, China aut NATIONALLICENCE 700 1- Xing Lijun Department of Otorhinolaryngology, The People's Hospital of Rushan City, 264500, Weihai, Shandong Province, China aut NATIONALLICENCE 700 1- Zhang Zhiqiang Department of Gastroenterology and Hepatology, People's Hospital of Huangdao, 266400, Qingdao, Shandong Province, China aut NATIONALLICENCE 700 1- Chen Lei Department of Otorhinolaryngology, The General Hospital of People's Liberation Army, No.28, Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2839-2845 0937-4477 272:10<2839 2015 272 405