caa a22 4500 606185283 CHVBK 20210128100831.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-014-3262-5 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3262-5 Development of hypoparathyroidism animal model and the feasibility of small intestinal submucosa application on the parathyroid autotransplantation [Elektronische Daten] [Hae Park, Soo Jung, Ha Kim, Da Kim, Moon Kim, Sung Chung, Young-Soo Rho, Han Kim] The purpose of this study is to evaluate the feasibility of small intestinal submucosa (SIS) application on the parathyroid autotransplantation in a rat model of hypoparathyroidism. The rats were divided into four groups: NC (no procedure, n=5), PTX (total parathyroidectomy, n=6), PT (total parathyroidectomy and parathyroid autotransplantation, n=10) and PT+SIS group (total parathyroidectomy and parathyroid autotransplantation with SIS, n=10). The levels of parathyroid hormone (PTH), calcium, and phosphorous were measured on 0, 3, 7, 21, 56 and 84days after surgery. PTH level was expressed as median (interquartile range) and histological and immunohistochemical examinations were performed. PTH levels were significantly decreased to "not detectable level” from day 3 in PTX group. PTH was not detected in both PT and PT+SIS groups on the 21st day. On the 56th day, PTH levels were increased in both groups: 3 out of 8 rats (37.5%) in the PT group, 6 out of 9 rats (66.7%) in the PT+SIS group. The PTH level was fully recovered to its preoperative range on the day 84 as 6 of 8 rats (75%) of the PT group and 7 of 9 rats (77.8%) of the PT+SIS group were recovered; the PTH levels were 117.84 and 178.36pg/ml, respectively. The neo-vascularization was well observed around the parathyroid tissue, and the number of new vessels formed was higher in the PT+SIS group (15 vessels/high power field) as compared to the PT group (10 vessels/high power field). This study showed the feasibility and the treatment effect of SIS as the success rate of autotransplantation of parathyroid tissue was significantly increased without severe inflammatory response in hypothyroidism animal model. Springer-Verlag Berlin Heidelberg, 2014 Animal model nationallicence Hypoparathyroidism nationallicence Small intestinal submucosa nationallicence Transplantation nationallicence Park Hae Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut Jung Soo Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut Kim Ha Molecular Medicine, School of Medicine, Ewha Womans University, Seoul, Korea aut Kim Da Department of Molecular Science and Technology, Ajou University, Suwon, Korea aut Kim Moon Department of Molecular Science and Technology, Ajou University, Suwon, Korea aut Chung Sung Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut Rho Young-Soo Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut Kim Han Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2969-2977 0937-4477 272:10<2969 2015 272 405 https://doi.org/10.1007/s00405-014-3262-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3262-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Hae Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut NATIONALLICENCE 700 1- Jung Soo Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Ha Molecular Medicine, School of Medicine, Ewha Womans University, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Da Department of Molecular Science and Technology, Ajou University, Suwon, Korea aut NATIONALLICENCE 700 1- Kim Moon Department of Molecular Science and Technology, Ajou University, Suwon, Korea aut NATIONALLICENCE 700 1- Chung Sung Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut NATIONALLICENCE 700 1- Rho Young-Soo Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Han Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, 158-710, Seoul, Korea aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2969-2977 0937-4477 272:10<2969 2015 272 405