caa a22 4500 606185313 CHVBK 20210128100831.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-014-3158-4 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3158-4 Decreased calcium-activated potassium channels by hypoxia causes abnormal firing in the spontaneous firing medial vestibular nuclei neurons [Elektronische Daten] [Hong Xie, Yu-qin Zhang, Xin-liang Pan, Shu-hui Wu, Xiang Chen, Jie Wang, Hua Liu, Xiao-zhong Qian, Zhi-guo Liu, Lie-Ju Liu] Vertebrobasilar insufficiency (VBI) presents complex varied clinical symptoms, including vertigo and hearing loss. Little is known, however, about how Ca2+-activated K+ channel attributes to the medial vestibular nucleus (MVN) neural activity in VBI. To address this issue, we performed whole-cell patch clamp and quantitative polymerase chain reaction (qPCR) to examine the effects of hypoxia on neural activity and the changes of the large conductance Ca2+ activated K+ channels (BKCa channels) in the MVN neurons in brain slices of male C57BL/6 mice. Brief hypoxic stimuli of the brain slices containing MVN were administrated by switching the normoxic artificial cerebrospinal fluid (ACSF) equilibrated with 21% O2/5% CO2 to hypoxic ACSF equilibrated with 5% O2/5% CO2 (balance N2). 3-min hypoxia caused a depolarization in the resting membrane potential (RM) in 8/11 non-spontaneous firing MVN neurons. 60/72 spontaneous firing MVN neurons showed a dramatic increase in firing frequency and a depolarization in the RM following brief hypoxia. The amplitude of the afterhyperpolarization (AHPA) was significantly decreased in both type A and type B spontaneous firing MVN neurons. Hypoxia-induced firing response was alleviated by pretreatment with NS1619, a selective BKCa activator. Furthermore, brief hypoxia caused a decrease in the amplitude of iberiotoxin-sensitive outward currents and mRNA level of BKCa in MVN neurons. These results suggest that BKCa channels protect against abnormal MVN neuronal activity induced by hypoxia, and might be a key target for treatment of vertigo and hearing loss in VBI. Springer-Verlag Berlin Heidelberg, 2014 Hypoxia nationallicence Calcium-activated potassium channels nationallicence Whole-cell recording nationallicence Medial vestibular nucleus nationallicence Slice nationallicence BKCa : Large conductance calcium-activated potassium channels nationallicence VBI : Vertebrobasilar insufficiency nationallicence MVN : Medial vestibular nucleus nationallicence ACSF : Artificial cerebrospinal fluid nationallicence IBTX : Iberiotoxin nationallicence Xie Hong Jingzhou Central Hospital, 434020, Jingzhou, People's Republic of China aut Zhang Yu-qin Department of Neurology, Anhui No.2 Provincial People's Hospital, 230041, Hefei, People's Republic of China aut Pan Xin-liang Department of Otolaryngology, Head and Neck Surgery, Qilu Hospital, Shandong University, Culture west road, 250012, Jinan, People's Republic of China aut Wu Shu-hui Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut Chen Xiang Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut Wang Jie Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut Liu Hua Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut Qian Xiao-zhong Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut Liu Zhi-guo Institution of Nutrition, Wuhan Polytechnic University, Changqing Garden, 430032, Wuhan, People's Republic of China aut Liu Lie-Ju Institution of Nutrition, Wuhan Polytechnic University, Changqing Garden, 430032, Wuhan, People's Republic of China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2703-2711 0937-4477 272:10<2703 2015 272 405 https://doi.org/10.1007/s00405-014-3158-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3158-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xie Hong Jingzhou Central Hospital, 434020, Jingzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yu-qin Department of Neurology, Anhui No.2 Provincial People's Hospital, 230041, Hefei, People's Republic of China aut NATIONALLICENCE 700 1- Pan Xin-liang Department of Otolaryngology, Head and Neck Surgery, Qilu Hospital, Shandong University, Culture west road, 250012, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Wu Shu-hui Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiang Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jie Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Hua Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Qian Xiao-zhong Department of Otolaryngology, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, You yi road, 201900, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Zhi-guo Institution of Nutrition, Wuhan Polytechnic University, Changqing Garden, 430032, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Lie-Ju Institution of Nutrition, Wuhan Polytechnic University, Changqing Garden, 430032, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2703-2711 0937-4477 272:10<2703 2015 272 405