caa a22 4500 60618547X CHVBK 20210128100832.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-014-3213-1 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3213-1 Hyaluronan up-regulation is linked to renal dysfunction and hearing loss induced by silver nanoparticles [Elektronische Daten] [Hao Feng, Ilmari Pyykkö, Jing Zou] Increased application of silver nanoparticles (AgNPs) has raised concerns on their potential adverse effects on human health. However, the precise toxicological mechanisms are not known in detail. The current study hypothesized that AgNPs induced glycosaminoglycan accumulation in the basement membrane that associated with the up-regulation of its component hyaluronic acid, known as a hydrophilic molecule of binding and retaining water, and caused toxicities in the kidney and cochlea. Rats administered AgNPs through either intravenous or intratympanic injection were observed at different time points after exposure. The concentrations of creatinine and urea in the serum were elevated remarkably, and proteins leaked into the urine were increased. A significant hearing loss over a broad range of frequencies was indicated. AgNP exposure induced glycosaminoglycan accumulation and hyaluronic acid up-regulation in the basement membrane. Abundant apoptotic cell death was demonstrated in the AgNP-exposed organs. Our results suggested that glycosaminoglycan accumulation associated with the up-regulation of hyaluronic acid was involved in the toxicities of kidney and cochlea caused by AgNPs. Springer-Verlag Berlin Heidelberg, 2014 Silver nanoparticles nationallicence Basement membrane nationallicence Hyaluronic acid nationallicence Glycosaminoglycan nationallicence Kidney nationallicence Cochlea nationallicence Feng Hao Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut Pyykkö Ilmari Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut Zou Jing Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2629-2642 0937-4477 272:10<2629 2015 272 405 https://doi.org/10.1007/s00405-014-3213-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3213-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Hao Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut NATIONALLICENCE 700 1- Pyykkö Ilmari Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut NATIONALLICENCE 700 1- Zou Jing Hearing and Balance Research Unit, Field of Oto-laryngology, School of Medicine, University of Tampere, Medisiinarinkatu 3, Room C2165, 33520, Tampere, Finland aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2629-2642 0937-4477 272:10<2629 2015 272 405