caa a22 4500 606185615 CHVBK 20210128100832.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00405-015-3664-z doi (NATIONALLICENCE)springer-10.1007/s00405-015-3664-z Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation [Elektronische Daten] [Xiaoxia Qiu, Donghui Chen, Meng Li, Yingna Gao, Fei Liu, Hongliang Zheng, Shicai Chen] The objective of this study was to investigate the myofiber subtype transition of human posterior cricoarytenoid (PCA) muscle after the injury to recurrent laryngeal nerve (RLN). PCA muscle specimens were obtained from 38 bilateral vocal fold paralysis patients underwent arytenoidectomy. According to the duration of RLN injury, all the cases were divided into five denervation groups: 6-12months, 1-2, 2-3, 3-6, and >6years. The normal PCA muscles from total laryngectomy patients were chosen as controls. Immunofluorescence was adopted to detect the expression level of myosin heavy chain (MHC)-I and MHC-II in PCA muscle. Quantitative real-time PCR was also used to assess the transcriptional level of MHC subtypes (MHC-I, MHC-IIa, MHC-IIb, MHC-IIx, embryonic-MHC, and peri-natal-MHC). Immunofluorescence showed that MHC-I-positive myofibers in denervation groups were much lower than control group, respectively, while MHC-II-positive myofibers were significantly higher than control group (P<0.05). With the extension of denervation, the number of MHC-I-positive myofibers gradually decreased, while MHC-II gradually increased and peaked in 1- to 2-year group. Transcriptional level of MHC-I, MHC-IIa, and MHC-IIb in denervation groups significantly down-regulated compared with the control (P<0.05), respectively. However, MHC-IIx, embryonic-MHC, and peri-natal-MHC significantly up-regulated in all denervation groups, and the highest level was in 1- to 2-year denervation group. Data from the present study demonstrated that the maximum transition of MHC subtypes in human PCA muscles occurred in 1-2years after denervation, suggesting that laryngeal reinnervation before the occurrence of irreversible transition of MHC subtypes could maintain the structural integrity of laryngeal PCA muscles. Springer-Verlag Berlin Heidelberg, 2015 Recurrent laryngeal nerve nationallicence Vocal fold paralysis nationallicence Posterior cricoarytenoid muscle nationallicence Myosin heavy chain nationallicence Laryngeal denervation nationallicence Qiu Xiaoxia Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Chen Donghui Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Li Meng Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Gao Yingna Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Liu Fei Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Zheng Hongliang Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut Chen Shicai Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2915-2923 0937-4477 272:10<2915 2015 272 405 https://doi.org/10.1007/s00405-015-3664-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-015-3664-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiu Xiaoxia Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Chen Donghui Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Li Meng Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Gao Yingna Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Liu Fei Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Zheng Hongliang Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Chen Shicai Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, 200433, Shanghai, China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/10(2015-10-01), 2915-2923 0937-4477 272:10<2915 2015 272 405