caa a22 4500 606185666 CHVBK 20210128100832.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00405-014-3165-5 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3165-5 Effect of nasal sprays on an in vitro survival and morphology of nasoseptal cartilage [Elektronische Daten] [Katharina Stoelzel, Benjamin Kohl, Mariann Hoyer, Carola Meier, Agnieszka Szczepek, Heidi Olze, Gundula Schulze-Tanzil] Nasal sprays were introduced several years ago to support the treatment of allergic rhinitis. These sprays may come in direct contact with directly exposed nasoseptal cartilage (e.g. is case of nasoseptal perforation). To date, no studies investigated the effects of nasal sprays on cartilage tissues and cells. Therefore, our aim was to analyze the influence of two different nasal spray types (thixotropic and liposomal) on the vitality of nasoseptal chondrocytes. Human chondrocytes were isolated from surgically dissected tissues. Alternatively, nasal septa (porcine and human) tissue explants were used. The cell or explant cultures were treated with nasal sprays for 4-24h. As a read-out, cell vitality and gene and protein expression profiles of type I and II collagen, SOX 9 and matrix metalloproteinase MMP-1 were compared to the untreated controls by means of real-time RT-PCR and immunostaining. Using the liposomal, but not thixotropic nasal spray in an explant or chondrocyte in vitro culture led to increased cell death, as compared to the untreated controls. A trend towards suppression of type II collagen and SOX 9 on protein level was found in cultures exposed to liposomal nasal spray, as compared to the controls. The thixotropic nasal spray has not affected the nasoseptal chondrocytes. Further studies with the use of viable nasoseptal cartilage explants and particularly using an in vivo animal model of exposed nasoseptal cartilage are necessary to clear the effect of liposomal spray on chondrocytes. Springer-Verlag Berlin Heidelberg, 2014 Allergic rhinitis nationallicence Thixotropic nasal spray nationallicence Liposomal nasal spray nationallicence Nasoseptal chondrocytes nationallicence Cell viability nationallicence MMP-1 : Matrix metalloproteinase-1 nationallicence NS-A : Thixotropic nasal spray nationallicence NS-B : Liposomal nasal spray nationallicence Stoelzel Katharina Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut Kohl Benjamin Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut Hoyer Mariann Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut Meier Carola Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut Szczepek Agnieszka Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut Olze Heidi Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut Schulze-Tanzil Gundula Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/4(2015-04-01), 877-887 0937-4477 272:4<877 2015 272 405 https://doi.org/10.1007/s00405-014-3165-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3165-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stoelzel Katharina Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Kohl Benjamin Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Hoyer Mariann Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Meier Carola Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Szczepek Agnieszka Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Olze Heidi Department of Otorhinolaryngology, Head and Neck Surgery, Charité-Universitätsmedizin, Campus Charité Mitte, Chariteplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Schulze-Tanzil Gundula Department of Orthopedic, Trauma and Reconstructive Surgery, Charité-Universitätsmedizin, Campus Benjamin Franklin, Garystrasse 5, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/4(2015-04-01), 877-887 0937-4477 272:4<877 2015 272 405