caa a22 4500 60618628X CHVBK 20210128100835.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00405-014-3008-4 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3008-4 Expression of TNF-α , OPG , IL-1β and the presence of the measles virus RNA in the stapes of the patients with otosclerosis [Elektronische Daten] [Małgorzata Potocka-Bakłażec, Monika Sakowicz-Burkiewicz, Jerzy Kuczkowski, Tadeusz Pawełczyk, Czesław Stankiewicz, Wojciech Sierszeń, Zbigniew Jankowski, Jacek Buczny] Persistent measles virus infections play a crucial role in the pathomechanism of otosclerosis. The study was undertaken to investigate the role of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and osteoprotegerin (OPG) in otosclerotic bone remodeling and to assess the relation of TNF-α, OPG and IL-1β expression levels in otosclerotic stape footplates to the occurrence of measles virus infection. 61 patients with otosclerosis were treated surgically. Thirty-one stapes obtained from cadavers of people, who had died from a sudden cause were used as a control group. The presence of measles virus RNA and the expression levels of TNF-α, IL-1β and OPG in otosclerotic foci were assessed using one-step RT-PCR. The presence of measles virus RNA was noted in 80.3 % of otosclerotic stapes (49 out of 61) and 9.7% of normal tissues (3 out of 31). Transcript of TNF-α, IL-1β and OPG was detected in 40, 46 and 18 virus-positive stapes, respectively. The transcript level of TNF-α and IL-1β was significantly higher in otosclerotic tissues comparing to normal tissue. The OPG expression level was significantly lower in otosclerotic tissues comparing to controls. The presence of measles virus RNA in the stapes may indicate its role in the pathogenesis of otosclerosis. The presence of TNF-α and IL-1β mRNA in the virus-positive stapes could be the result of viral antigen stimulation and may be a marker of inflammation the otosclerotic focus. The lack of OPG mRNA and the presence of TNF-α and IL-1β mRNA in the majority of otosclerotic tissues reflect the bone remodeling process occurring in the stapes. The Author(s), 2014 Otosclerosis nationallicence Etiopathogenesis nationallicence Measles virus nationallicence Cytokines nationallicence Potocka-Bakłażec Małgorzata Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut Sakowicz-Burkiewicz Monika Department of Molecular Medicine, Medical University of Gdańsk, Gdańsk, Poland aut Kuczkowski Jerzy Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut Pawełczyk Tadeusz Department of Molecular Medicine, Medical University of Gdańsk, Gdańsk, Poland aut Stankiewicz Czesław Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut Sierszeń Wojciech Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut Jankowski Zbigniew Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland aut Buczny Jacek University of Social Sciences and Humanities, Sopot, Poland aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/8(2015-08-01), 1907-1912 0937-4477 272:8<1907 2015 272 405 https://doi.org/10.1007/s00405-014-3008-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3008-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Potocka-Bakłażec Małgorzata Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut NATIONALLICENCE 700 1- Sakowicz-Burkiewicz Monika Department of Molecular Medicine, Medical University of Gdańsk, Gdańsk, Poland aut NATIONALLICENCE 700 1- Kuczkowski Jerzy Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut NATIONALLICENCE 700 1- Pawełczyk Tadeusz Department of Molecular Medicine, Medical University of Gdańsk, Gdańsk, Poland aut NATIONALLICENCE 700 1- Stankiewicz Czesław Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut NATIONALLICENCE 700 1- Sierszeń Wojciech Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland aut NATIONALLICENCE 700 1- Jankowski Zbigniew Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland aut NATIONALLICENCE 700 1- Buczny Jacek University of Social Sciences and Humanities, Sopot, Poland aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/8(2015-08-01), 1907-1912 0937-4477 272:8<1907 2015 272 405