caa a22 4500 606186433 CHVBK 20210128100836.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00405-014-3191-3 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3191-3 Azelastine enhances the clinical efficacy of glucocorticoid by modulating MKP-1 expression in allergic rhinitis [Elektronische Daten] [Xi Luo, Renqiang Ma, Xingmei Wu, Desheng Xian, Jian Li, Zhonglin Mou, Huabin Li] Azelastine was suggested as a supplementary choice of glucocorticoid for the control of moderate to severe allergic rhinitis (AR). However, the underlying mechanism has not been completely understood. In this study, primary cultured nasal epithelial cells and bronchial epithelial cells were stimulated with proinflammatory cytokines (IL-1β and IL-17A) and anti-inflammatory agents (azelastine and budesonide) in vitro. The expression of intercellular adhesion molecule 1 (ICAM-1) and mitogen-activated protein kinase phosphatase-1 (MKP-1) was examined using qPCR and ELISA, respectively. Moreover, the additive effects of azelastine and budesonide nasal spray on nasal ICAM-1 level and total nasal symptom scores were evaluated in six uncontrolled severe AR patients by budesonide nasal spray alone. We found azelastine significantly inhibited cytokine-induced ICAM-1 upregulation, which is reversed by MKP-1 silencing. Azelastine and budesonide additively increased MKP-1 expression and inhibited ICAM-1 expression in vitro. After treatment for two consecutive weeks, combined azelastine and budesonide nasal spray significantly decreased nasal ICAM-1 level and TNSS in six uncontrolled AR patients. Our findings suggested that azelastine is able to additively enhance the anti-inflammatory effect of budesonide by modulating MKP-1 expression, which may implicate in the treatment of uncontrolled severe AR. Springer-Verlag Berlin Heidelberg, 2014 Allergic rhinitis nationallicence Epithelial cells nationallicence ICAM-1 nationallicence Azelastine nationallicence Budesonide nationallicence MKP-1 nationallicence Luo Xi Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut Ma Renqiang Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut Wu Xingmei Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut Xian Desheng Department of Otolaryngology, Affiliated Hospital of Hainan Medical University, No. 31, Longhua Road, 570102, Haikou, China aut Li Jian Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut Mou Zhonglin Department of Otolaryngology, Affiliated Hospital of Hainan Medical University, No. 31, Longhua Road, 570102, Haikou, China aut Li Huabin Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/5(2015-05-01), 1165-1173 0937-4477 272:5<1165 2015 272 405 https://doi.org/10.1007/s00405-014-3191-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3191-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Xi Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Ma Renqiang Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Wu Xingmei Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Xian Desheng Department of Otolaryngology, Affiliated Hospital of Hainan Medical University, No. 31, Longhua Road, 570102, Haikou, China aut NATIONALLICENCE 700 1- Li Jian Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Mou Zhonglin Department of Otolaryngology, Affiliated Hospital of Hainan Medical University, No. 31, Longhua Road, 570102, Haikou, China aut NATIONALLICENCE 700 1- Li Huabin Allergy and Cancer Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, No. 58, Zhongshan 2nd Road, 510080, Guangzhou, China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/5(2015-05-01), 1165-1173 0937-4477 272:5<1165 2015 272 405