caa a22 4500 606186557 CHVBK 20210128100836.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00405-014-3176-2 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3176-2 Establishment and characterization of an experimental mouse model of allergic rhinitis [Elektronische Daten] [Ming-Tse Ko, Shun-Chen Huang, Hong-Yo Kang] Allergic rhinitis (AR) is a common worldwide disease. Animal studies on AR were adopted in various investigations. However, animal studies simply aimed at establishing an animal model for AR have been seldom seen. The purpose of this study was to introduce an easy-to-establish experimental mouse model of AR. To develop a mouse model of AR, 38 Balb/c mice were randomly assigned to two groups. Mice in the study group were sensitized by intraperitoneal (IP) injection of ovalbumin (OVA) on day 1 and 6, followed by continuous inhalation (IH) of OVA solution for 1week (day 8-14) using a newly designed inhalation box. The control group mice received sensitization of IP normal saline and IH sterilized distilled water instead of OVA. Before and after sensitization, the frequencies of nasal symptoms (sneezing, nasal rubbing) were recorded and the serum levels of total immunoglobulin E (IgE) were evaluated using ELISA. Finally, the murine nasal mucosal tissues were stained by Giemsa solution to estimate the degree of mast cell infiltration. After sensitization by IP and IH OVA, the study group showed significant phenotypic changes including increased sneezing and rubbing. Pathological and cytological findings also confirmed significant elevated serum total IgE titer and local mast cell infiltration in the study group statistically. We successfully developed a workable experimental animal model for AR that was more easily sensitized using our new-designed inhalation box, with less stress and more precisely to be observed. Springer-Verlag Berlin Heidelberg, 2014 Allergic rhinitis nationallicence Animal model nationallicence Ovalbumin nationallicence Inhalation nationallicence Ko Ming-Tse Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut Huang Shun-Chen Department of Anatomic Pathology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut Kang Hong-Yo Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/5(2015-05-01), 1149-1155 0937-4477 272:5<1149 2015 272 405 https://doi.org/10.1007/s00405-014-3176-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3176-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ko Ming-Tse Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut NATIONALLICENCE 700 1- Huang Shun-Chen Department of Anatomic Pathology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut NATIONALLICENCE 700 1- Kang Hong-Yo Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Kaohsiung, Taiwan aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/5(2015-05-01), 1149-1155 0937-4477 272:5<1149 2015 272 405