caa a22 4500 606186778 CHVBK 20210128100837.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00405-014-3112-5 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3112-5 Polyhexanide-containing solution reduces ciliary beat frequency of human nasal epithelial cells in vitro [Elektronische Daten] [Richard Birk, C. Aderhold, J. Stern-Sträter, K. Hörmann, B. Stuck, J. Sommer] In ENT, polyhexanide-containing solutions are used to treat nasal infections caused by multiresistant bacteria like methicillin-resistant Staphylococcus aureus. Many forms of commercial nasal solutions containing polyhexanide exist, such as gels or solutions for topical use. Data regarding the influence of polyhexanide on ciliary beat frequency (CBF) are lacking to date. We tested the CBF of nasal ciliated epithelial cells under the influence of a commercially available polyhexanide-containing solution (Lavasept® Concentrate) in a therapeutic concentration (0.04, 0.02%). In addition, we tested the concentrations of 0.1 and 0.01%. Cells were visualized with a phase contrast microscope, and the CBF was measured with the SAVA system's region of interest method. Ringer's solution and macrogol served as negative controls. A therapeutic concentration of Lavasept significantly reduced CBF in a time- and concentration-dependent manner. After 1min, the CBF was reduced from 8.90±1.64 to 5.00±3.72Hz with a concentration of 0.04% (p value=0.001). After 10min, all cilia stopped beating. After 5min, a 0.02% solution of Lavasept concentrate decreased CBF significantly from 8.64±1.71 to 3.30±3.27Hz (p value<0.001). In conclusion, CBF of human nasal epithelia is significantly reduced with the use of the polyhexanide-containing solution Lavasept in some therapeutic concentrations. Due to our findings in this study, Lavasept should be used on ciliated mucosa only with caution and in a concentration of 0.02%. Springer-Verlag Berlin Heidelberg, 2014 Polyhexanide nationallicence Cilia beat frequency nationallicence Nasal mucosa nationallicence CBF nationallicence Birk Richard Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut Aderhold C. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut Stern-Sträter J. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut Hörmann K. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut Stuck B. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut Sommer J. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/2(2015-02-01), 377-383 0937-4477 272:2<377 2015 272 405 https://doi.org/10.1007/s00405-014-3112-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3112-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Birk Richard Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Aderhold C. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Stern-Sträter J. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Hörmann K. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Stuck B. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Sommer J. Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/2(2015-02-01), 377-383 0937-4477 272:2<377 2015 272 405