caa a22 4500 606187421 CHVBK 20210128100840.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00405-014-3477-5 doi (NATIONALLICENCE)springer-10.1007/s00405-014-3477-5 Expression and distribution of epithelial sodium channel in nasal polyp and nasal mucosa [Elektronische Daten] [Yiming Jiang, Ji Xu, Yanqing Chen, Jiali Shi, Chun Zhang, Jiping Li] To evaluate the expression and location of epithelial sodium channels (ENaCs) in human nasal polyp and normal nasal mucosa, and to characterize the relevance of ENaCs to the development of NPs. Nasal polyp tissue from 17 patients and nasal mucosa from ten patients were obtained through endoscopic sinus surgery. The mRNA concentrations of ENaC-α, β, and γ were detected by real-time polymerase chain reaction. The expression of ENaC-α was detected using western blot and immunofluorescence techniques. The distribution of ENaC-α in mucosal tissue was observed using a laser scanning confocal microscope. The transcriptional expression of three subunits of ENaC was in the following order: α>β>γ, in both groups. The transcriptional expression of α, β, and γ subunits of ENaC was elevated in nasal polyp compared to nasal mucosa (p<0.01). ENaC-α expression was higher in nasal polyp than in nasal mucosa (p<0.05). Immunofluorescent staining indicated that ENaC-α protein was distributed in the epithelial cell apical membrane. The expression of ENaC is upregulated in human nasal polyp, which might in turn facilitate the formation and development of nasal polyp. Springer-Verlag Berlin Heidelberg, 2015 Nasal polyp nationallicence Epithelial cells nationallicence Epithelial sodium channel nationallicence ENaC-mediated absorption nationallicence Jiang Yiming Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut Xu Ji Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut Chen Yanqing Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut Shi Jiali Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut Zhang Chun Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut Li Jiping Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/11(2015-11-01), 3361-3366 0937-4477 272:11<3361 2015 272 405 https://doi.org/10.1007/s00405-014-3477-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00405-014-3477-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Yiming Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Xu Ji Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yanqing Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Shi Jiali Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chun Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Jiping Department of Otorhinolaryngology Head and Neck Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pu Jian Road, 200127, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer Berlin Heidelberg 272/11(2015-11-01), 3361-3366 0937-4477 272:11<3361 2015 272 405