caa a22 4500 606190287 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9457-1 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9457-1 TALEN-mediated genome engineering to generate targeted mice [Elektronische Daten] [Daniel Sommer, Annika Peters, Ann-Kathrin Baumgart, Marc Beyer] Genetic mouse models are critical for biomedical research to understand gene function and pathophysiology. In the last years, the generation of genetic mouse models has been revolutionized by the emergence of transcription activator-like effector nucleases (TALENs). TALENs are programmable, sequence-specific DNA-binding proteins fused to a non-specific endonuclease domain used as powerful tools for site-specific induction of DNA double-strand breaks. These result in disruption of the gene product of the targeted locus by mutations induced during repair by error-prone non-homologous end-joining. Alternatively, these DNA double-strand breaks can be exploited to integrate a user-defined sequence by homologous recombination if an appropriate repair plasmid is provided. In this review, we highlight the major technological improvements for genome editing in murine oocytes which have been achieved using TALENs, discuss current limitations of the technology, suggest strategies to broadly apply TALENs, and describe possible future directions to facilitate gene editing in murine oocytes. Springer Science+Business Media Dordrecht, 2015 TALEN nationallicence transcription activator-like effector nucleases nationallicence genome engineering nationallicence transgenic animals nationallicence oocyte injections nationallicence DSB : DNA double-strand break nationallicence ES cells : Embryonic stem cells nationallicence HDR : Homology-directed repair nationallicence HR : Homologous recombination nationallicence ICA : Iterative capped assembly nationallicence LIC : Ligation-independent cloning nationallicence NHEJ : Non-homologous end-joining nationallicence ODN : Oligodeoxynucleotide nationallicence RVD : Repeat-variable di-residue nationallicence SSA : Single-stranded annealing nationallicence TAL : Transcription activator-like nationallicence TALENs : Transcription activator-like effector nucleases nationallicence USER : Uracil-specific excision reagent nationallicence ZFN : Zinc-finger nuclease nationallicence CRISPR : Clustered regularly interspaced short palindromicrepeats nationallicence Sommer Daniel LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut Peters Annika LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut Baumgart Ann-Kathrin LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut Beyer Marc LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut Chromosome Research Springer Netherlands 23/1(2015-02-01), 43-55 0967-3849 23:1<43 2015 23 10577 https://doi.org/10.1007/s10577-014-9457-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9457-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sommer Daniel LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Peters Annika LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Baumgart Ann-Kathrin LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Beyer Marc LIMES-Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 43-55 0967-3849 23:1<43 2015 23 10577