caa a22 4500 606190295 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9458-0 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9458-0 Katona Robert Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary aut De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications [Elektronische Daten] [Robert Katona] Mammalian artificial chromosomes (MACs) are non-integrating, autonomously replicating natural chromosome-based vectors that may carry a vast amount of genetic material, which in turn enable potentially prolonged, safe, and regulated therapeutic transgene expression and render MACs as attractive genetic vectors for "gene replacement” or for controlling differentiation pathways in target cells. Satellite-DNA-based artificial chromosomes (SATACs) can be made by induced de novo chromosome formation in cells of different mammalian and plant species. These artificially generated accessory chromosomes are composed of predictable DNA sequences, and they contain defined genetic information. SATACs have already passed a number of obstacles crucial to their further development as gene therapy vectors, including large-scale purification, transfer of purified artificial chromosomes into different cells and embryos, generation of transgenic animals and germline transmission with purified SATACs, and the tissue-specific expression of a therapeutic gene from an artificial chromosome in the milk of transgenic animals. SATACs could be used in cell therapy protocols. For these methods, the most versatile target cell would be one that was pluripotent and self-renewing to address multiple disease target cell types, thus making multilineage stem cells, such as adult derived early progenitor cells and embryonic stem cells, as attractive universal host cells. Springer Science+Business Media Dordrecht, 2014 Cell therapy nationallicence Stem cell nationallicence Recombinase nationallicence Integrase nationallicence Transgene nationallicence ACEs : Artificial chromosome expression system nationallicence CHO : Chinese hamster ovary nationallicence EB : Embryoid body nationallicence EGFP : Enhanced green fluorescent protein nationallicence ESC : Embryonic stem cell nationallicence EPO : Erythropoietin nationallicence FACS : Fluorescence-activated cell sorting nationallicence HAC : Human artificial chromosome nationallicence hESC : Human embryonic stem cell nationallicence HGH : Human growth hormone nationallicence HPRT : Hypoxanthine guanine phosphoribosyl transferase nationallicence HSC : Hematopoietic Stem Cell nationallicence iPS : Induced pluripotent stem nationallicence MAbs : Monoclonal antibodies nationallicence MACs : Mammalian artificial chromosomes nationallicence MMCT : Microcell-mediated chromosome transfer nationallicence MRI : Magnetic resonance imaging nationallicence MSC : Mesenchymal stem cell nationallicence PET : Positron emission tomography nationallicence SATAC : Satellite-DNA-based artificial chromosome nationallicence Chromosome Research Springer Netherlands 23/1(2015-02-01), 143-157 0967-3849 23:1<143 2015 23 10577 https://doi.org/10.1007/s10577-014-9458-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9458-0 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Katona Robert Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 143-157 0967-3849 23:1<143 2015 23 10577