caa a22 4500 606190309 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9454-4 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9454-4 Birchler James Division of Biological Sciences, 311 Tucker Hall, University of Missouri, 65211, Columbia, MO, USA aut Engineered minichromosomes in plants [Elektronische Daten] [James Birchler] Engineered minichromosomes have been produced in several plant species via telomere-mediated chromosomal truncation. This approach bypasses the complications of the epigenetic nature of centromere function in plants, which has to date precluded the production of minichromosomes by the re-introduction of centromere sequences to a plant cell. Genes to be added to a cleaved chromosome are joined together with telomere repeats on one side. When these constructs are introduced into plant cells, the genes are ligated to the broken chromosomes but the telomere repeats will catalyze the formation of a telomere on the other end cutting the chromosome at that point. Telomere-mediated chromosomal truncation is sufficiently efficient that very small chromosomes can be generated consisting of basically the endogenous centromere and the added transgenes. The added transgenes provide a platform onto which it should be possible to assemble a synthetic chromosome to specification. Combining engineered minichromosomes with doubled haploid breeding should greatly expedite the transfer of transgenes to new lines and to test the interaction of transgenes in new background genotypes. Potential basic and applied applications of synthetic chromosomes are discussed. Springer Science+Business Media Dordrecht, 2014 Artificial chromosomes nationallicence Synthetic biology nationallicence B chromosomes nationallicence Telomeres nationallicence Genome editing nationallicence Genetic engineering nationallicence Centromeres nationallicence Epigenetics nationallicence Gene stacking nationallicence CENH3 : Variant of histone H3 present at active centromeres nationallicence CRM2 : Centromeric retrotransposon maize 2 nationallicence BiBAC : Binary bacterial artificial chromosome vector for plant transformation nationallicence Chromosome Research Springer Netherlands 23/1(2015-02-01), 77-85 0967-3849 23:1<77 2015 23 10577 https://doi.org/10.1007/s10577-014-9454-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9454-4 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Birchler James Division of Biological Sciences, 311 Tucker Hall, University of Missouri, 65211, Columbia, MO, USA aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 77-85 0967-3849 23:1<77 2015 23 10577