caa a22 4500 606190341 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9451-7 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9451-7 Advances in genetic modification of farm animals using zinc-finger nucleases (ZFN) [Elektronische Daten] [Bjoern Petersen, Heiner Niemann] Genome editing tools (GET), including zinc-finger nucleases (ZFN), transcription activator-like endonucleases (TALENS), and meganucleases possess long recognition sites and are thus capable of cutting DNA in a very specific manner. These genome editing tools mediate targeted genetic alterations by enhancing DNA mutation frequency via induction of double-strand breaks at a predetermined genomic site. Compared to conventional homologous recombination based gene targeting, GETs can increase gene targeting and gene disruption via mutagenic DNA repair more than 10,000-fold. Recently, a novel class of genome editing tools was described that uses RNAs to target a specific genomic site. The CRISPR/Cas9 system is capable of targeting even multiple genomic sites in one shot and thus could be superior to ZFNs or TALEN. Current results indicate that these tools can be successfully employed in a broad range of organisms which renders them useful for improving the understanding of complex physiological systems, producing transgenic animals, including creating large animal models for human diseases, creating specific cell lines, and plants, and even for treating human genetic diseases. This review provides an update on the use of ZFNs to modify the genome of farm animals, summarizes current knowledge on the underlying mechanism, and discusses new opportunities for generating genetically modified farm animals. Springer Science+Business Media Dordrecht, 2014 Zinc-Finger Nucleases nationallicence Farm animals nationallicence Gene targeting nationallicence Genome editing nationallicence CRISPR : Clustered regularly interspaced short palindromic repeats nationallicence DSB : Double-strand break nationallicence EGFP : Enhanced green fluorescent protein nationallicence FACS : Fluorescence-activated cell sorting nationallicence GET : Genome editing tools nationallicence HR : Homologous recombination nationallicence HDR : Homology-directed repair nationallicence IPS : Induced pluripotent stem cells nationallicence NHEJ : Nonhomologous end joining nationallicence SCNT : Somatic cell nuclear transfer nationallicence SSB : Single-strand break nationallicence TALEN : Transcription activator-like effector endonuclease nationallicence ZF : Zinc-finger nationallicence ZFN : Zinc-finger nuclease nationallicence Petersen Bjoern Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Neustadt a. Rbge, Hoeltystrasse 10, Mariensee, 31535, Neustadt, Germany aut Niemann Heiner Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Neustadt a. Rbge, Hoeltystrasse 10, Mariensee, 31535, Neustadt, Germany aut Chromosome Research Springer Netherlands 23/1(2015-02-01), 7-15 0967-3849 23:1<7 2015 23 10577 https://doi.org/10.1007/s10577-014-9451-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9451-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petersen Bjoern Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Neustadt a. Rbge, Hoeltystrasse 10, Mariensee, 31535, Neustadt, Germany aut NATIONALLICENCE 700 1- Niemann Heiner Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Neustadt a. Rbge, Hoeltystrasse 10, Mariensee, 31535, Neustadt, Germany aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 7-15 0967-3849 23:1<7 2015 23 10577