caa a22 4500 60619035X CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9450-8 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9450-8 Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes [Elektronische Daten] [Justin Overcash, Azadeh Aryan, Kevin Myles, Zach Adelman] Mosquitoes are high-impact disease vectors with the capacity to transmit pathogenic agents that cause diseases such as malaria, yellow fever, chikungunya, and dengue. Continued growth in knowledge of genetic, molecular, and physiological pathways in mosquitoes allows for the development of novel control methods and for the continued optimization of existing ones. The emergence of site-specific nucleases as genomic engineering tools promises to expedite research of crucial biological pathways in these disease vectors. The utilization of these nucleases in a more precise and efficient manner is dependent upon knowledge and manipulation of the DNA repair pathways utilized by the mosquito. While progress has been made in deciphering DNA repair pathways in some model systems, research into the nature of the hierarchy of mosquito DNA repair pathways, as well as in mechanistic differences that may exist, is needed. In this review, we will describe progress in the use of site-specific nucleases in mosquitoes, along with the hierarchy of DNA repair in the context of mosquito chromosomal organization and structure, and how this knowledge may be manipulated to achieve precise chromosomal engineering in mosquitoes. Springer Science+Business Media Dordrecht, 2014 Mosquito nationallicence Aedes nationallicence Gene editing nationallicence TALEN nationallicence CRISPR nationallicence DNA repair nationallicence A-NHEJ : Alternative non-homologous end joining nationallicence C-NHEJ : Classical non-homologous end joining nationallicence CRISPR : Clustered regulatory interspaced short palindromic repeats nationallicence DSB : Double-stranded break nationallicence HE : Homing endonuclease nationallicence HDR : Homology-directed repair nationallicence HRMA : High-resolution melt curve analysis nationallicence RIDL : Release of insects with dominant lethality nationallicence SIT : Sterile insect technique nationallicence SSA : Single-strand annealing nationallicence TALEN : Transcription activator-like effector nuclease nationallicence TE : Transposable element nationallicence ZFN : Zinc finger nuclease nationallicence Overcash Justin Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut Aryan Azadeh Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut Myles Kevin Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut Adelman Zach Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut Chromosome Research Springer Netherlands 23/1(2015-02-01), 31-42 0967-3849 23:1<31 2015 23 10577 https://doi.org/10.1007/s10577-014-9450-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9450-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Overcash Justin Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Aryan Azadeh Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Myles Kevin Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Adelman Zach Fralin Life Science Institute and Department of Entomology, Virginia Tech, 305 Fralin Life Science Institute, 360 West Campus Dr., 24061, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 31-42 0967-3849 23:1<31 2015 23 10577