caa a22 4500 606190368 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-015-9470-z doi (NATIONALLICENCE)springer-10.1007/s10577-015-9470-z Genetic and epigenetic regulation of centromeres: a look at HAC formation [Elektronische Daten] [Jun-ichirou Ohzeki, Vladimir Larionov, William Earnshaw, Hiroshi Masumoto] The centromere is a specialized chromosomal locus required for accurate chromosome segregation. A specific histone H3 variant, CENP-A, assembles at centromeres. CENP-A is required for kinetochore protein assembly and is an epigenetic marker for the maintenance of a functional centromere. Human CENP-A chromatin normally assembles on α-satellite DNA (alphoid DNA), a centromeric repetitive sequence. Using alphoid DNA arrays, human artificial chromosomes (HACs) have been constructed in human HT1080 cells and used to dissect the requirements for CENP-A assembly on DNA sequence. However, centromere formation is not a simple genetic event. In other commonly used human cell lines, such as HeLa and U2OS cells, no functional de novo centromere formation occurs efficiently with the same centromeric alphoid DNA sequences. Recent studies using protein tethering combined with the HAC system and/or genetic manipulation have revealed that epigenetic chromatin regulation mechanisms are also involved in the CENP-A chromatin assembly pathway and subsequent centromere/kinetochore formation. We summarize the DNA sequence requirements for CENP-A assembly and discuss the epigenetic regulation of human centromeres. Springer Science+Business Media Dordrecht, 2015 Centromere nationallicence CENP-A nationallicence HAC nationallicence Kinetochore nationallicence Heterochromatin nationallicence CENP-B nationallicence HAT nationallicence Alphoid DNA : Alpha satellite DNA nationallicence ARS : Autonomous replicating sequence nationallicence CAD : CENP-A distal nationallicence CCAN : Constitutive centromere-associated network nationallicence CDE : Conserved DNA element nationallicence CENP : Centromere protein nationallicence HAC : Human artificial chromosome nationallicence HOR : Higher order repeat nationallicence ICEN : Interphase Centromere Complex nationallicence KMN : KNL-1/Mis12 complex/Ndc80 nationallicence NAC : CENP-A nucleosome-associated complex nationallicence YAC : Yeast artificial chromosome nationallicence Ohzeki Jun-ichirou Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, 292-0818, Kisarazu, Chiba, Japan aut Larionov Vladimir Developmental Therapeutics Branch, National Cancer Institute, National Institute of Health, Bethesda, MD, USA aut Earnshaw William Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK aut Masumoto Hiroshi Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, 292-0818, Kisarazu, Chiba, Japan aut Chromosome Research Springer Netherlands 23/1(2015-02-01), 87-103 0967-3849 23:1<87 2015 23 10577 https://doi.org/10.1007/s10577-015-9470-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9470-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohzeki Jun-ichirou Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, 292-0818, Kisarazu, Chiba, Japan aut NATIONALLICENCE 700 1- Larionov Vladimir Developmental Therapeutics Branch, National Cancer Institute, National Institute of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Earnshaw William Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK aut NATIONALLICENCE 700 1- Masumoto Hiroshi Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, 292-0818, Kisarazu, Chiba, Japan aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 87-103 0967-3849 23:1<87 2015 23 10577