caa a22 4500 606190384 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10577-014-9449-1 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9449-1 Engineering of plant chromosomes [Elektronische Daten] [Michael Mette, Andreas Houben] Engineered minimal chromosomes with sufficient mitotic and meiotic stability have an enormous potential as vectors for stacking multiple genes required for complex traits in plant biotechnology. Proof of principle for essential steps in chromosome engineering such as truncation of chromosomes by T-DNA-mediated telomere seeding and de novo formation of centromeres by cenH3 fusion protein tethering has been recently obtained. In order to generate robust protocols for application in plant biotechnology, these steps need to be combined and supplemented with additional methods such as site-specific recombination for the directed transfer of multiple genes of interest on the minichromosomes. At the same time, the development of these methods allows new insight into basic aspects of plant chromosome functions such as how centromeres assure proper distribution of chromosomes to daughter cells or how telomeres serve to cap the chromosome ends to prevent shortening of ends over DNA replication cycles and chromosome end fusion. Springer Science+Business Media Dordrecht, 2014 Minichromosome nationallicence Telomere seeding nationallicence De novo centromere nationallicence Double-strand break nationallicence B chromosome nationallicence BFB : Breakage-fusion-bridge nationallicence cenH3 : Centromeric histone H3-variant nationallicence Cre : Phage P1 recombinase nationallicence CRISPR : Clustered regularly interspaced short palindromic repeats nationallicence DSB : Double-strand break nationallicence GFP : Green fluorescence protein nationallicence LacI : Lac inhibitor protein nationallicence lacO : Lac operator sequence nationallicence loxP : Locus of crossing-over on phage P1 nationallicence PARC : Plant artificial ring chromosome nationallicence Mette Michael Research Group Chromosome Structure and Function, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland OT Gatersleben, Germany aut Houben Andreas Research Group Chromosome Structure and Function, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland OT Gatersleben, Germany aut Chromosome Research Springer Netherlands 23/1(2015-02-01), 69-76 0967-3849 23:1<69 2015 23 10577 https://doi.org/10.1007/s10577-014-9449-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9449-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mette Michael Research Group Chromosome Structure and Function, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland OT Gatersleben, Germany aut NATIONALLICENCE 700 1- Houben Andreas Research Group Chromosome Structure and Function, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland OT Gatersleben, Germany aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/1(2015-02-01), 69-76 0967-3849 23:1<69 2015 23 10577