caa a22 4500 606190406 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10577-015-9481-9 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9481-9 The puzzling character of repetitive DNA in Phodopus genomes (Cricetidae, Rodentia) [Elektronische Daten] [Ana Paço, Filomena Adega, Nevenka Meštrović, Miroslav Plohl, Raquel Chaves] Three novel repetitive DNA sequences are described, presenting a similar heterochromatic chromosomal location in two hamster species: Phodopus roborovskii and Phodopus sungorus (Cricetidae, Rodentia). Namely, two species-specific repetitive sequences (PROsat from P. roborovskii and PSUchr1sat from P. sungorus) surrounding a third one (PsatDNA), that is shared by both hamster genomes. Fiber-FISH analyses revealed that PROsat intermingles with PsatDNA in P. roborovskii and PSUchr1sat intermingles with PsatDNA in P. sungorus. A model explaining the evolution of this intricate chromosomal distribution is proposed, which can explain better the evolution of these very derivative genomes (in comparison to the ancestral Muroidea). The most plausible evolutionary scenario seems to be the expansion of a number of repeats into other's domain, most probably resulting in its intermingling, followed by the subsequent spread of these complex repeats from a single chromosomal location to other chromosomes. Evidences of an association between repetitive sequences and the chromosome evolution process were observed, namely for PROsat. Most probably, the evolutionary breakpoints that shaped PRO and PSU chromosomes (pericentric inversions and fusions) occurred within the boundaries of PROsat blocks in the ancestor. The repeats high diversity at the heterochromatic regions of Phodopus chromosomes, together with its complex organization, suggests that these species are important models for evolutionary studies, namely in the investigation of a possible relationship between repetitive sequences and the occurrence of chromosomal rearrangements and consequently, in genome evolution. Springer Science+Business Media Dordrecht, 2015 Rodentia nationallicence Phodopus nationallicence Repetitive sequences nationallicence Chromosomal evolution nationallicence 3′UTR : 3′ untranslated region nationallicence 5′TAMRA : 5′ Carboxytetramethylrhodamine nationallicence APK : Ancestral Phodopus Karyotype nationallicence BLAST : Basic Local Alignment Search Tool nationallicence CBP-banding : C-bands by barium hydroxide with propidium iodide nationallicence CCR : Cricetus cricetus nationallicence EMBOSS : European Molecular Biology Open Software Suite nationallicence Fiber-FISH : FISH on stretched chromatin fibers nationallicence FISH : fluorescent in situ hybridization nationallicence FITC : fluorescein isothiocyanate nationallicence LINE-1 : long interspersed nuclear element-1 nationallicence NCBI : National Center for Biotechnology Information nationallicence ORF2 : open reading frame 2 nationallicence PCR : polymerase chain reaction nationallicence PER : Peromyscus eremicus nationallicence PRO : Phodopus roborovskii nationallicence PROsat : Phodopus roborovskii satellite nationallicence PsatDNA : Phodopus satellite DNA nationallicence PSU : Phodopus sungorus nationallicence PSUchr1sat : Phodopus sungorus chromosome 1 satellite nationallicence REs : restriction enzymes nationallicence RNO : Rattus norvegicus nationallicence SatDNAs : Satellite DNAs nationallicence Paço Ana University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut Adega Filomena University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut Meštrović Nevenka Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10002, Zagreb, Croatia aut Plohl Miroslav Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10002, Zagreb, Croatia aut Chaves Raquel University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut Chromosome Research Springer Netherlands 23/3(2015-09-01), 427-440 0967-3849 23:3<427 2015 23 10577 https://doi.org/10.1007/s10577-015-9481-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9481-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paço Ana University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut NATIONALLICENCE 700 1- Adega Filomena University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut NATIONALLICENCE 700 1- Meštrović Nevenka Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10002, Zagreb, Croatia aut NATIONALLICENCE 700 1- Plohl Miroslav Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10002, Zagreb, Croatia aut NATIONALLICENCE 700 1- Chaves Raquel University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics and Biotechnology (DGB), Laboratory of Cytogenomics and Animal Genomics (CAG), Vila Real, Portugal aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/3(2015-09-01), 427-440 0967-3849 23:3<427 2015 23 10577