caa a22 4500 606190481 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10577-015-9485-5 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9485-5 Three-dimensional architecture of tandem repeats in chicken interphase nucleus [Elektronische Daten] [Antonina Maslova, Anna Zlotina, Nadezhda Kosyakova, Marina Sidorova, Alla Krasikova] Tandem repeats belong to a class of genomic repetitive elements that form arrays of head-to-tail monomers. Due to technical difficulties in sequencing and assembly of large tandem repeat arrays, it remains largely unknown by which mechanisms tandem-repeat-containing regions aid in maintenance of ordered radial genome organization during interphase. Here we analyzed spatial distribution of several types of tandem repeats in interphase nuclei of chicken MDCC-MSB1 cells and somatic tissues relative to heterochromatin compartments and nuclear center. We showed that telomere and subtelomere repeats generally localize at the nuclear or chromocenters periphery. A tandem repeat known as CNM, typical for centromere regions of gene-dense microchromosomes, forms interchromosome clusters and occupies DAPI-positive chromocenters that appear predominantly within the nuclear interior. In contrast, centromere-specific tandem repeats of the majority of gene-poor macrochromosomes are embedded into the peripheral layer of heterochromatin. Chicken chromocenters rarely comprise centromere sequences of both macro- and microchromosomes, whose territories localize in different radial nuclear zones. Possible mechanisms of observed tandem repeats positioning and its implication in highly ordered arrangement of chromosome territories in chicken interphase nucleus are discussed. Springer Science+Business Media Dordrecht, 2015 Centromere nationallicence Chicken genome nationallicence Chromocenters nationallicence Genome architecture nationallicence Heterochromatin nationallicence Interphase nucleus nationallicence MDCC-MSB1 nationallicence Subtelomere nationallicence Tandem repeats nationallicence Telomere nationallicence 3C : Chromosome conformation capture nationallicence 3D : Three-dimensional nationallicence BAC : Bacterial artificial chromosome nationallicence Cen : Centromere-specific sequence nationallicence CENP : Centromere protein nationallicence CEF : Chicken embryonic fibroblasts nationallicence CNM : Chicken nuclear-membrane-associated repeat nationallicence CR1 : Chicken repetitive 1 element nationallicence DAPI : 4′,6-Diamidino-2-phenylindole nationallicence FISH : Fluorescent in situ hybridization nationallicence GGA : Chicken (Gallus gallus) chromosome nationallicence LAD : Lamina-associated domain nationallicence MDCC : Marek's disease chicken cell line nationallicence PO41 : Pattern of 41bp repeat nationallicence Maslova Antonina Saint Petersburg State University, 198504, Saint Petersburg, Russia aut Zlotina Anna Saint Petersburg State University, 198504, Saint Petersburg, Russia aut Kosyakova Nadezhda Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany aut Sidorova Marina Saint Petersburg State University, 198504, Saint Petersburg, Russia aut Krasikova Alla Saint Petersburg State University, 198504, Saint Petersburg, Russia aut Chromosome Research Springer Netherlands 23/3(2015-09-01), 625-639 0967-3849 23:3<625 2015 23 10577 https://doi.org/10.1007/s10577-015-9485-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9485-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maslova Antonina Saint Petersburg State University, 198504, Saint Petersburg, Russia aut NATIONALLICENCE 700 1- Zlotina Anna Saint Petersburg State University, 198504, Saint Petersburg, Russia aut NATIONALLICENCE 700 1- Kosyakova Nadezhda Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany aut NATIONALLICENCE 700 1- Sidorova Marina Saint Petersburg State University, 198504, Saint Petersburg, Russia aut NATIONALLICENCE 700 1- Krasikova Alla Saint Petersburg State University, 198504, Saint Petersburg, Russia aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/3(2015-09-01), 625-639 0967-3849 23:3<625 2015 23 10577