caa a22 4500 60619052X CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10577-015-9480-x doi (NATIONALLICENCE)springer-10.1007/s10577-015-9480-x Helitrons shaping the genomic architecture of Drosophila [Elektronische Daten] enrichment of DINE-TR1 in α- and β-heterochromatin, satellite DNA emergence, and piRNA expression [Guilherme Dias, Pedro Heringer, Marta Svartman, Gustavo Kuhn] Drosophila INterspersed Elements (DINEs) constitute an abundant but poorly understood group of Helitrons present in several Drosophila species. The general structure of DINEs includes two conserved blocks that may or not contain a region with tandem repeats in between. These central tandem repeats (CTRs) are similar within species but highly divergent between species. It has been assumed that CTRs have independent origins. Herein, we identify a subset of DINEs, termed DINE-TR1, which contain homologous CTRs of approximately 150bp. We found DINE-TR1 in the sequenced genomes of several Drosophila species and in Bactrocera tryoni (Acalyptratae, Diptera). However, interspecific high sequence identity (∼88%) is limited to the first ∼30bp of each tandem repeat, implying that evolutionary constraints operate differently over the monomer length. DINE-TR1 is unevenly distributed across the Drosophila phylogeny. Nevertheless, sequence analysis suggests vertical transmission. We found that CTRs within DINE-TR1 have independently expanded into satellite DNA-like arrays at least twice within Drosophila. By analyzing the genome of Drosophila virilis and Drosophila americana, we show that DINE-TR1 is highly abundant in pericentromeric heterochromatin boundaries, some telomeric regions and in the Y chromosome. It is also present in the centromeric region of one autosome from D. virilis and dispersed throughout several euchromatic sites in both species. We further found that DINE-TR1 is abundant at piRNA clusters, and small DINE-TR1-derived RNA transcripts (∼25nt) are predominantly expressed in the testes and the ovaries, suggesting active targeting by the piRNA machinery. These features suggest potential piRNA-mediated regulatory roles for DINEs at local and genome-wide scales in Drosophila. Springer Science+Business Media Dordrecht, 2015 Drosophila virilis nationallicence Drosophila americana nationallicence Transposable element nationallicence Genome evolution nationallicence Helitrons nationallicence AIC : Akaike Information Criterion nationallicence CTRs : Central tandem repeats nationallicence DINEs : Drosophila INterspersed Elements nationallicence FISH : Fluorescence in situ hybridization nationallicence IR : Inverted repeat nationallicence miRNA : microRNA nationallicence MSL : Male-specific lethal complex nationallicence piRNA : Piwi-interacting RNA nationallicence satDNA : Satellite DNA nationallicence siRNA : Small interfering RNA nationallicence SPR : Subtree pruning and regrafting algorithm nationallicence subTIRs : Subterminal inverted repeats nationallicence TE : Transposable element nationallicence TR : Tandem repeat nationallicence Dias Guilherme Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut Heringer Pedro Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut Svartman Marta Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut Kuhn Gustavo Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut Chromosome Research Springer Netherlands 23/3(2015-09-01), 597-613 0967-3849 23:3<597 2015 23 10577 https://doi.org/10.1007/s10577-015-9480-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9480-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dias Guilherme Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut NATIONALLICENCE 700 1- Heringer Pedro Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut NATIONALLICENCE 700 1- Svartman Marta Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut NATIONALLICENCE 700 1- Kuhn Gustavo Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/3(2015-09-01), 597-613 0967-3849 23:3<597 2015 23 10577