caa a22 4500 606190538 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10577-015-9488-2 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9488-2 Miga Karen Center for Biomolecular Science and Engineering, University of California Santa Cruz, 95064, Santa Cruz, CA, USA aut Completing the human genome: the progress and challenge of satellite DNA assembly [Elektronische Daten] [Karen Miga] Genomic studies rely on accurate chromosome assemblies to explore sequence-based models of cell biology, evolution and biomedical disease. However, even the extensively studied human genome has not yet reached a complete, ‘telomere-to-telomere', chromosome assembly. The largest assembly gaps remain in centromeric regions and acrocentric short arms, sites known to contain megabase-sized arrays of tandem repeats, or satellite DNAs. This review aims to briefly address the progress and challenges of generating correct assemblies of satellite DNA arrays. Although the focus is placed on the human genome, many concepts presented here are applicable to other genomes. Springer Science+Business Media Dordrecht, 2015 Satellite DNA nationallicence assembly nationallicence centromere nationallicence pericentromeric heterochromatin nationallicence acrocentric repeats nationallicence HVGM : Human Variation Genome Map nationallicence ONT : Oxford Nanopore Technology nationallicence PacBio : Pacific Biosciences nationallicence rDNA : Ribosomal DNA nationallicence WGS : Whole genome shotgun nationallicence Chromosome Research Springer Netherlands 23/3(2015-09-01), 421-426 0967-3849 23:3<421 2015 23 10577 https://doi.org/10.1007/s10577-015-9488-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9488-2 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Miga Karen Center for Biomolecular Science and Engineering, University of California Santa Cruz, 95064, Santa Cruz, CA, USA aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/3(2015-09-01), 421-426 0967-3849 23:3<421 2015 23 10577