caa a22 4500 606190546 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10577-015-9487-3 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9487-3 Transcription of subtelomere tandemly repetitive DNA in chicken embryogenesis [Elektronische Daten] [Irina Trofimova, Darya Chervyakova, Alla Krasikova] Transcription of tandemly repetitive DNA in embryogenesis seems to be of special interest due to a crucial role of non-coding RNAs in many aspects of development. However, only a few data are available on tandem repeats transcription at subtelomere regions of chromosomes during vertebrate embryogenesis. To reduce this gap, we examined stage and tissue-specific pattern of subtelomeric PO41 (pattern of 41bp) tandem repeat transcription during embryogenesis of chicken (Gallus gallus domesticus). Using whole-mount RNA fluorescent in situ hybridization and reverse transcription PCR with specific primers, we demonstrated that both strands of PO41 repeat are transcribed at each of the studied stages of chicken embryo development: from 7-8 HH to 20 HH stages. Subtelomere-derived transcripts localize in the nuclei of all cell types and throughout the all embryonic bodies: head, somites, tail, wings and buds. In embryo-dividing cells and cultured embryonic fibroblasts, PO41 RNAs envelop terminal regions of chromosomes. PO41-containing RNAs are predominantly single-stranded and can be polyadenylated, indicating appearance of non-nascent form of subtelomeric transcripts. PO41 repeat RNAs represent a rare example of ubiquitously transcribed non-coding RNAs, such as Xist/XIST RNA or telomere repeat-containing RNA. Distribution of PO41 repeat transcripts at different stages of embryo development and among cell types has extremely uniform pattern, indicating on possible universal functions of PO41 non-coding RNAs. Springer Science+Business Media Dordrecht, 2015 Chicken nationallicence Embryogenesis nationallicence Mitosis nationallicence Non-coding RNA nationallicence Subtelomere nationallicence Tandem repeat nationallicence Transcription nationallicence Whole-mount in situ hybridization nationallicence CEFs : Chicken embryonic fibroblasts nationallicence HH : Hamburger and Hamilton nationallicence DMEM : Dulbecco's modified Eagle's medium nationallicence PO41 : Pattern of 41bp nationallicence RNA FISH : RNA fluorescent in situ hybridization nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence WISH : Whole-mount in situ hybridization nationallicence Trofimova Irina Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut Chervyakova Darya Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut Krasikova Alla Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut Chromosome Research Springer Netherlands 23/3(2015-09-01), 495-503 0967-3849 23:3<495 2015 23 10577 https://doi.org/10.1007/s10577-015-9487-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9487-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trofimova Irina Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut NATIONALLICENCE 700 1- Chervyakova Darya Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut NATIONALLICENCE 700 1- Krasikova Alla Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/3(2015-09-01), 495-503 0967-3849 23:3<495 2015 23 10577