caa a22 4500 606190589 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10577-015-9500-x doi (NATIONALLICENCE)springer-10.1007/s10577-015-9500-x Next-generation sequencing reveals differentially amplified tandem repeats as a major genome component of Northern Europe's oldest Camellia japonica [Elektronische Daten] [Tony Heitkam, Stefan Petrasch, Falk Zakrzewski, Anja Kögler, Torsten Wenke, Stefan Wanke, Thomas Schmidt] Northern Europe's oldest and largest Camellia japonica growing at the Pillnitz Castle (Germany) for over 200years is of botanical and cultural importance and is a reference for C. japonica molecular scale analysis. In order to provide a fundament for genome analysis of the genus Camellia, we characterize the C. japonica tandem repeat fraction, constituting 12.5% of the Pillnitz camellia's genome. A genomic library of the Pillnitz C. japonica was produced and Illumina sequenced to generate 36Gb of paired-end reads. We performed graph-based read clustering implemented in the RepeatExplorer pipeline to estimate the C. japonica repeat fraction of 73%. This enabled us to identify and characterize the most prominent satellite DNAs, Camellia japonica satellite 1-4 (CajaSat1-CajaSat4), and the 5S ribosomal DNA (rDNA) by bioinformatics, fluorescent in situ and Southern hybridization. Within the Camellia genus, satellite spreading, array expansion and formation of higher-order structures highlight different modes of repeat evolution. The CajaSat satellites localize at prominent chromosomal sites, including (peri)centromeres and subtelomeres of all chromosomes, thus serving as chromosomal landmarks for their identification. This work provides an insight into the C. japonica chromosome organization and significantly expands the Camellia genomic knowledge, also with respect to the tea plant Camellia sinensis. Springer Science+Business Media Dordrecht, 2015 Camellia japonica nationallicence RepeatExplorer nationallicence Fluorescent in situ hybridization nationallicence Satellite DNA nationallicence Tandem repeats nationallicence BAC : Bacterial artificial chromosome nationallicence bp : Base pair nationallicence BSA : Bovine serum albumin nationallicence CajaSat : Camellia japonica satellite nationallicence cDNA : Copy DNA nationallicence Cy3 : Cyanine 3 nationallicence DAPI : 4′,6′-Diamidino-2-phenylindole nationallicence FISH : Fluorescent in situ hybridization nationallicence FITC : Fluorescein isothiocyanate nationallicence Gb : Gigabase nationallicence LINE : Long interspersed nuclear element nationallicence LTR : Long terminal repeat nationallicence PCR : Polymerase chain reaction nationallicence rDNA : Ribosomal DNA nationallicence rRNA : Ribosomal RNA nationallicence SDS : Sodium dodecyl sulfate nationallicence SSC : Saline sodium citrate (1× SSC = 0.15M NaCl + 0.015M Na3 citrate) nationallicence Heitkam Tony Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Petrasch Stefan Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Zakrzewski Falk Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Kögler Anja Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Wenke Torsten Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Wanke Stefan Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Schmidt Thomas Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut Chromosome Research Springer Netherlands 23/4(2015-12-01), 791-806 0967-3849 23:4<791 2015 23 10577 https://doi.org/10.1007/s10577-015-9500-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9500-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heitkam Tony Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Petrasch Stefan Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Zakrzewski Falk Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Kögler Anja Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Wenke Torsten Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Wanke Stefan Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Schmidt Thomas Institute of Botany, Technische Universität Dresden, 01069, Dresden, Germany aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/4(2015-12-01), 791-806 0967-3849 23:4<791 2015 23 10577