caa a22 4500 606190619 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10577-015-9477-5 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9477-5 Preferential recruitment of the maternal centromere-specific histone H3 (CENH3) in oat ( Avena sativa L.) × pearl millet ( Pennisetum glaucum L.) hybrid embryos [Elektronische Daten] [Takayoshi Ishii, Naohiro Sunamura, Ayaka Matsumoto, Amin Eltayeb, Hisashi Tsujimoto] Chromosome elimination occurs frequently in interspecific hybrids between distantly related species in Poaceae. However, chromosomes from both parents behave stably in a hybrid of female oat (Avena sativa L.) pollinated by pearl millet (Pennisetum glaucum L.). To analyze the chromosome behavior in this hybrid, we cloned the centromere-specific histone H3 (CENH3) genes of oat and pearl millet and produced a pearl millet-specific anti-CENH3 antibody. Application of this antibody together with a grass species common anti-CENH3 antibody revealed the dynamic CENH3 composition of the hybrid cells before and after fertilization. Despite co-expression of CENH3 genes encoded by oat and pearl millet, only an oat-type CENH3 was incorporated into the centromeres of both species in the hybrid embryo. Oat CENH3 enables a functional centromere in pearl millet chromosomes in an oat genetic background. Comparison of CENH3 genes among Poaceae species that show chromosome elimination in interspecific hybrids revealed that the loop 1 regions of oat and pearl millet CENH3 exhibit exceptionally high similarity. Springer Science+Business Media Dordrecht, 2015 oat nationallicence pearl millet nationallicence embryogenesis nationallicence centromere-specific histone H3 (CENH3) nationallicence interspecific cross nationallicence chromosome elimination nationallicence CENH3 : Centromere-specific histone H3 nationallicence FISH : Fluorescence in situ hybridization nationallicence Anti- Pg CENH3 : Pearl millet CENH3-specific antibody nationallicence Anti-GrassCENH3 : Poaceae CENH3-specific antibody nationallicence Ishii Takayoshi Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut Sunamura Naohiro Faculty of Medicine, Tottori University, 683-8503, Yonago, Tottori, Japan aut Matsumoto Ayaka Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut Eltayeb Amin Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut Tsujimoto Hisashi Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut Chromosome Research Springer Netherlands 23/4(2015-12-01), 709-718 0967-3849 23:4<709 2015 23 10577 https://doi.org/10.1007/s10577-015-9477-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9477-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishii Takayoshi Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut NATIONALLICENCE 700 1- Sunamura Naohiro Faculty of Medicine, Tottori University, 683-8503, Yonago, Tottori, Japan aut NATIONALLICENCE 700 1- Matsumoto Ayaka Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut NATIONALLICENCE 700 1- Eltayeb Amin Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut NATIONALLICENCE 700 1- Tsujimoto Hisashi Arid Land Research Center, Tottori University, 680-0001, Hamasaka, Tottori, Japan aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/4(2015-12-01), 709-718 0967-3849 23:4<709 2015 23 10577