caa a22 4500 606190686 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9448-2 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9448-2 Sex-specific recombination maps for individual macrochromosomes in the Japanese quail ( Coturnix japonica ) [Elektronische Daten] [Lucía del Priore, María Pigozzi] Meiotic recombination in the Japanese quail was directly studied by immunolocalization of mutL homolog 1 (MLH1), a mismatch repair protein of mature recombination nodules. In total, 15,862 crossovers were scored along the autosomal synaptonemal complexes in 308 meiotic nuclei from males and females. Crossover frequencies calculated from MLH1 foci show wide similitude between males and females with slightly higher number of foci in females. From this analysis, we predict that the sex-averaged map length of the Japanese quail is 2580cM, with a genome-wide recombination rate of 1.9cM/Mb. MLH1 focus mapping along the six largest bivalents showed few intersex differences in the distribution of crossovers along with variant patterns in metacentric and acrocentric macrobivalents. These results provide valuable information to complement linkage map analysis in the species while providing insight into our understanding of the mechanisms of crossover distribution along chromosome arms. Springer Science+Business Media Dordrecht, 2015 Birds nationallicence Meiosis nationallicence Crossing over nationallicence MLH1 foci nationallicence Poultry genome nationallicence Japanese quail nationallicence MLH1 : mutL homolog 1 nationallicence SC : Synaptonemal complex nationallicence cM : Centimorgan nationallicence SMC3 : Structural maintenance of chromosomes 3 nationallicence CREST : Calcinosis, Raynaud phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasia nationallicence RN : Recombination nodule nationallicence del Priore Lucía INBIOMED (CONICET-UBA), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 Piso 10, C1121ABG, Buenos Aires, Argentina aut Pigozzi María INBIOMED (CONICET-UBA), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 Piso 10, C1121ABG, Buenos Aires, Argentina aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 199-210 0967-3849 23:2<199 2015 23 10577 https://doi.org/10.1007/s10577-014-9448-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9448-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- del Priore Lucía INBIOMED (CONICET-UBA), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 Piso 10, C1121ABG, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Pigozzi María INBIOMED (CONICET-UBA), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 Piso 10, C1121ABG, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 199-210 0967-3849 23:2<199 2015 23 10577