caa a22 4500 606190694 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-015-9468-6 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9468-6 Low rate of interchromosomal rearrangements during old radiation of gekkotan lizards (Squamata: Gekkota) [Elektronische Daten] [Martina Pokorná, Vladimir Trifonov, Willem Rens, Malcolm Ferguson-Smith, Lukáš Kratochvíl] Gekkotan lizards are a highly specious (∼1600 described species) clade of squamate lizards with nearly cosmopolitan distribution in warmer areas. The clade is primarily nocturnal and forms an ecologically dominant part of the world nocturnal herpetofauna. However, molecular cytogenetic methods to study the evolution of karyotypes have not been widely applied in geckos. Our aim here was to uncover the extent of chromosomal rearrangements across the whole group Gekkota and to search for putative synapomorphies supporting the newly proposed phylogenetic relationships within this clade. We applied cross-species chromosome painting with the recently derived whole-chromosomal probes from the gekkonid species Gekko japonicus to members of the major gekkotan lineages. We included members of the families Diplodactylidae, Carphodactylidae, Pygopodidae, Eublepharidae, Phyllodactylidae and Gekkonidae. Our study demonstrates relatively high chromosome conservatism across the ancient group of gekkotan lizards. We documented that many changes in chromosomal shape across geckos can be attributed to intrachromosomal rearrangements. The documented rearrangements are not totally in agreement with the recently newly erected family Phyllodactylidae. The results also pointed to homoplasy, particularly in the reuse of chromosome breakpoints, in the evolution of gecko karyotypes. Springer Science+Business Media Dordrecht, 2015 genome evolution nationallicence geckos nationallicence chromosome painting nationallicence karyotype evolution nationallicence GJA : Gekko japonicus nationallicence PCR : Polymerase chain reaction nationallicence qPCR : Quantitative polymerase chain reaction nationallicence DOP-PCR : Degenerate oligonucleotide primed PCR nationallicence FISH : Fluorescence in situ hybridization nationallicence dUTP : Deoxy-uridine-5′-triphosphate nationallicence SSC : Saline sodium citrate nationallicence DAPI : 4′,6-Diamidino-2-phenylindole nationallicence Pokorná Martina Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Praha 2, Czech Republic aut Trifonov Vladimir Institute of Molecular and Cellular Biology SB RAS, 630090, Novosibirsk, Russia aut Rens Willem Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK aut Ferguson-Smith Malcolm Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK aut Kratochvíl Lukáš Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Praha 2, Czech Republic aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 299-309 0967-3849 23:2<299 2015 23 10577 https://doi.org/10.1007/s10577-015-9468-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9468-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pokorná Martina Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Praha 2, Czech Republic aut NATIONALLICENCE 700 1- Trifonov Vladimir Institute of Molecular and Cellular Biology SB RAS, 630090, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Rens Willem Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK aut NATIONALLICENCE 700 1- Ferguson-Smith Malcolm Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK aut NATIONALLICENCE 700 1- Kratochvíl Lukáš Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Praha 2, Czech Republic aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 299-309 0967-3849 23:2<299 2015 23 10577