caa a22 4500 606190708 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9464-2 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9464-2 Intra- and interchromosomal rearrangements between cowpea [ Vigna unguiculata (L.) Walp.] and common bean ( Phaseolus vulgaris L.) revealed by BAC-FISH [Elektronische Daten] [Emanuelle Vasconcelos, Artur de Andrade Fonsêca, Andrea Pedrosa-Harand, Kyria de Andrade Bortoleti, Ana Benko-Iseppon, Antônio da Costa, Ana Brasileiro-Vidal] Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris ‘BAT93' BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes. Springer Science+Business Media Dordrecht, 2015 Bacterial artificial chromosome (BAC) nationallicence Fluorescent in situ hybridization (FISH) nationallicence macrosynteny nationallicence translocation nationallicence inversion nationallicence karyotype evolution nationallicence BAC : Bacterial artificial chromosome nationallicence CABMV : Cowpea aphid born mosaic virus nationallicence CPSMV : Cowpea severe mosaic virus nationallicence DAPI : 4′,6-Diamidino-2-phenylindole nationallicence FISH : Fluorescent in situ hybridization nationallicence LG : Linkage group nationallicence Mya : Million years ago nationallicence rDNA : Ribosomal DNA nationallicence SSC : Saline-sodium citrate nationallicence Vasconcelos Emanuelle Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut de Andrade Fonsêca Artur Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut Pedrosa-Harand Andrea Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut de Andrade Bortoleti Kyria Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco, 56304-917, Petrolina, PE, Brazil aut Benko-Iseppon Ana Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut da Costa Antônio Instituto Agronômico de Pernambuco, Av. General San Martin, 1371, CEP: 50761-000, Recife, PE, Brazil aut Brasileiro-Vidal Ana Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 253-266 0967-3849 23:2<253 2015 23 10577 https://doi.org/10.1007/s10577-014-9464-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9464-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vasconcelos Emanuelle Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- de Andrade Fonsêca Artur Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Pedrosa-Harand Andrea Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- de Andrade Bortoleti Kyria Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco, 56304-917, Petrolina, PE, Brazil aut NATIONALLICENCE 700 1- Benko-Iseppon Ana Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- da Costa Antônio Instituto Agronômico de Pernambuco, Av. General San Martin, 1371, CEP: 50761-000, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Brasileiro-Vidal Ana Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 253-266 0967-3849 23:2<253 2015 23 10577