caa a22 4500 606190732 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9461-5 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9461-5 Intragenomic distribution of RTE retroelements suggests intrachromosomal movement [Elektronische Daten] [Eugenia Montiel, Francisco Ruiz-Ruano, Josefa Cabrero, Juan Marchal, Antonio Sánchez, Francisco Perfectti, María López-León, Juan Camacho] Much is known about the abundance of transposable elements (TEs) in eukaryotic genomes, but much is still unknown on their behaviour within cells. We employ here a combination of cytological, molecular and genomic approaches providing information on the intragenomic distribution and behaviour of non-long terminal repeat (LTR) retrotransposon-like elements (RTE). We microdissected every chromosome in a single first meiotic metaphase cell of the grasshopper Eyprepocnemis plorans and polymerase chain reaction (PCR) amplified a fragment of the RTE reverse transcriptase gene with specific primers. PCR products were cloned and 139 clones were sequenced. Analysis of molecular variance (AMOVA) showed significant intragenomic structure for these elements, with 4.6% of molecular variance being found between chromosomes. A maximum likelihood tree built with the RTE sequences revealed the frequent presence of two or more elements showing very high similarity and being located on the same chromosome, thus suggesting intrachromosome movement. The 454 pyrosequencing of genomic DNA gave strong support to the microdissection results and provided evidence for the existence of 5′ truncated elements. Our results thus indicate a tendency of RTE elements to reinsert into the same chromosome from where they were transcribed, which could be achieved if retrotranscription and insertion takes place immediately after transcription. Springer Science+Business Media Dordrecht, 2014 454 sequencing nationallicence Intrachromosomal nationallicence Intragenomic nationallicence Microdissection nationallicence RTE nationallicence Transposable elements nationallicence AMOVA : Analysis of molecular variance nationallicence dNTP : Deoxynucleoside triphosphate nationallicence LINE : Long interspersed nuclear element nationallicence LTR : Long terminal repeat nationallicence ORF : Open reading frame nationallicence PCR : Polymerase chain reaction nationallicence RTE : Non-LTR retrotransposon-like element nationallicence SINE : Short interspersed nuclear element nationallicence TE : Transposable element nationallicence Montiel Eugenia Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut Ruiz-Ruano Francisco Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut Cabrero Josefa Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut Marchal Juan Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut Sánchez Antonio Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut Perfectti Francisco Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut López-León María Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut Camacho Juan Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 211-223 0967-3849 23:2<211 2015 23 10577 https://doi.org/10.1007/s10577-014-9461-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9461-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montiel Eugenia Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Ruiz-Ruano Francisco Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Cabrero Josefa Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Marchal Juan Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut NATIONALLICENCE 700 1- Sánchez Antonio Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut NATIONALLICENCE 700 1- Perfectti Francisco Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- López-León María Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Camacho Juan Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 211-223 0967-3849 23:2<211 2015 23 10577