caa a22 4500 606190740 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9442-8 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9442-8 Impact of the number of Robertsonian chromosomes on germ cell death in wild male house mice [Elektronische Daten] [Nuria Medarde, Valeria Merico, Mª López-Fuster, Maurizio Zuccotti, Silvia Garagna, Jacint Ventura] Previous studies in the house mouse have shown that the presence of Robertsonian (Rb) metacentric chromosomes in heterozygous condition affects the process of spermatogenesis. This detrimental effect mainly depends on the number of metacentrics involved and the complexity of the resulting meiotic figures. In this study, we aimed at elucidating the relationship between the chromosomal composition and spermatogenesis impairment in mice present in an area of chromosomal polymorphism (the so-called Barcelona system BRbS) in which Rb mice are surrounded by all acrocentric animals, no established metacentric races are present and the level of structural heterozygosity is relatively low. Using the terminal deoxinucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, we report higher frequency of apoptotic spermatogenetic cells in mice carrying six pairs of metacentrics at the homozygous state than in those carrying two or three fusions at the heterozygous state. Specifically, we detected a higher frequency of TUNEL-positive (T+) tubules and of T+ cells per tubule cross section and also a lower spermatid/spermatocyte ratio. These results indicate that the number of metacentrics at the homozygous state is more influential in determining apoptotic germ cell death than that of moderate chromosome heterozygosity. The percentage of germ cell death lower than 50% found in our samples and the geographic distribution of the set of metacentrics within the BRbS indicate that although the spermatogenic alterations detected in this area could act as a partial barrier to gene flow, they are not sufficient to prevent Rb chromosomes from spreading in nature. Springer Science+Business Media Dordrecht, 2015 Apoptosis nationallicence Spermatogenesis nationallicence Robertsonian fusion nationallicence Western house mouse nationallicence Mus musculus domesticus nationallicence Rb : Robertsonian nationallicence St : Standard nationallicence BRbS : Barcelona Rb System nationallicence Ks : Karyotype structure nationallicence 2 n : Diploid number nationallicence MM : Homozygous metacentrics for six metacentrics and heterozygous for none nationallicence H1-H3 : Heterozygous for one to three metacentrics and homozygous for zero to five nationallicence T+ : TUNEL positive nationallicence GCD : Germ cell death nationallicence RS : Round spermatids nationallicence PS : Pachytene spermatocytes nationallicence MI : Metaphase I nationallicence MII : Metaphase II nationallicence Medarde Nuria Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain aut Merico Valeria Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani”, Università degli Studi di Pavia, Via A. Ferrata, 9, 27100, Pavia, Italy aut López-Fuster Mª Departament de Biologia Animal and Institut de Recerca de la Biodiversitat (IRBio), Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut Zuccotti Maurizio Dipartimento di Science Biomediche, Biotecnologiche e Traslazionali (S.Bi.Bi.T.), Unità di Anatomia, Istologia ed Embriologia, Univesità degli Studi di Parma, Via Volturno 9, 43125, Parma, Italy aut Garagna Silvia Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani”, Università degli Studi di Pavia, Via A. Ferrata, 9, 27100, Pavia, Italy aut Ventura Jacint Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 159-169 0967-3849 23:2<159 2015 23 10577 https://doi.org/10.1007/s10577-014-9442-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9442-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Medarde Nuria Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain aut NATIONALLICENCE 700 1- Merico Valeria Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani”, Università degli Studi di Pavia, Via A. Ferrata, 9, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- López-Fuster Mª Departament de Biologia Animal and Institut de Recerca de la Biodiversitat (IRBio), Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Zuccotti Maurizio Dipartimento di Science Biomediche, Biotecnologiche e Traslazionali (S.Bi.Bi.T.), Unità di Anatomia, Istologia ed Embriologia, Univesità degli Studi di Parma, Via Volturno 9, 43125, Parma, Italy aut NATIONALLICENCE 700 1- Garagna Silvia Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani”, Università degli Studi di Pavia, Via A. Ferrata, 9, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Ventura Jacint Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 159-169 0967-3849 23:2<159 2015 23 10577