caa a22 4500 606190759 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9463-3 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9463-3 A cytogenetic and comparative map of camelid chromosome 36 and the minute in alpacas [Elektronische Daten] [Felipe Avila, Malorie Baily, David Merriwether, Vladimir Trifonov, Jiři Rubes, Michelle Kutzler, Renuka Chowdhary, Jan Janečka, Terje Raudsepp] Recent advances in camelid genomics have provided draft sequence assemblies and the first comparative and gene maps for the dromedary (CDR) and the alpaca (LPA). However, no map information is currently available for the smallest camelid autosome—chr36. The chromosome is also of clinical interest because of its involvement in the minute chromosome syndrome (MCS) in infertile alpacas. Here, we developed molecular markers for camelid chr36 by direct sequencing CDR36 and LPA minute and by bioinformatics analysis of alpaca unplaced sequence scaffolds. We constructed a cytogenetic map for chr36 in the alpaca, llama, and dromedary and showed its homology to human chromosome 7 (HSA7) at 49.8-55.5Mb. The chr36 map comprised seven markers, including two genes—ZPBP and WVC2. Comparative status of HSA7 was further refined by cytogenetic mapping of 16 HSA7 orthologs in camelid chromosomes 7 and 18 and by the analysis of HSA7-conserved synteny blocks across 11 vertebrate species. Finally, mapping chr36 markers in infertile alpacas confirmed that the minute chromosome was a derivative of chr36, but the small size was not a result of a large deletion or a translocation. Instead, cytogenetic mapping of 5.8S, 18S, and 28S rRNA genes (nucleolus organizer region (NOR)) revealed that the size difference between chr36 homologs in infertile alpacas was due to a heterozygous presence of NOR, whereas chr36 in fertile alpacas had no NOR. We theorized that the heterozygous NOR might affect chr36 pairing, recombination, and segregation in meiosis and, thus fertility. Springer Science+Business Media Dordrecht, 2015 Camelids nationallicence Chromosome 36 nationallicence HSA7 nationallicence Minute chromosome syndrome nationallicence Infertility nationallicence NOR nationallicence CBA : Bactrian camel (Camelus bactrianus) nationallicence CDR : Dromedary (Camelus dromedarius,) nationallicence EBR : Evolutionary breakpoint region nationallicence FISH : Fluorescence in situ hybridization nationallicence HSA : Human (Homo sapiens) nationallicence LGL : Llama (Lama glama) nationallicence LPA : Alpaca (Lama pacos) nationallicence MCS : Minute chromosome syndrome nationallicence NGS : Next-generation sequencing nationallicence NOR : Nucleolus organizer region nationallicence location of 18S, 28S, and 5.8S rRNA genes nationallicence RH : Radiation hybrid nationallicence rRNA : Ribosomal RNA nationallicence SMRT : Single molecule real time nationallicence Avila Felipe Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut Baily Malorie Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut Merriwether David Binghamton University (SUNY), Binghamton, NY, USA aut Trifonov Vladimir Institute of Molecular and Cellular Biology, SВ RAS, Novosibirsk, Russia aut Rubes Jiři Veterinary Research Institute, Brno, Czech Republic aut Kutzler Michelle Department of Animal and Rangeland Sciences, Oregon State University, 97331, Corvallis, OR, USA aut Chowdhary Renuka Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut Janečka Jan Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut Raudsepp Terje Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 237-251 0967-3849 23:2<237 2015 23 10577 https://doi.org/10.1007/s10577-014-9463-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9463-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Avila Felipe Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut NATIONALLICENCE 700 1- Baily Malorie Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut NATIONALLICENCE 700 1- Merriwether David Binghamton University (SUNY), Binghamton, NY, USA aut NATIONALLICENCE 700 1- Trifonov Vladimir Institute of Molecular and Cellular Biology, SВ RAS, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Rubes Jiři Veterinary Research Institute, Brno, Czech Republic aut NATIONALLICENCE 700 1- Kutzler Michelle Department of Animal and Rangeland Sciences, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Chowdhary Renuka Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut NATIONALLICENCE 700 1- Janečka Jan Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut NATIONALLICENCE 700 1- Raudsepp Terje Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 77843-4458, College Station, TX, USA aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 237-251 0967-3849 23:2<237 2015 23 10577