caa a22 4500 606190775 CHVBK 20210128100857.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9444-6 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9444-6 Comparative cytogenetic characterization of primary canine melanocytic lesions using array CGH and fluorescence in situ hybridization [Elektronische Daten] [Kelsey Poorman, Luke Borst, Scott Moroff, Siddharth Roy, Philippe Labelle, Alison Motsinger-Reif, Matthew Breen] Melanocytic lesions originating from the oral mucosa or cutaneous epithelium are common in the general dog population, with up to 100,000 diagnoses each year in the USA. Oral melanoma is the most frequent canine neoplasm of the oral cavity, exhibiting a highly aggressive course. Cutaneous melanocytomas occur frequently, but rarely develop into a malignant form. Despite the differential prognosis, it has been assumed that subtypes of melanocytic lesions represent the same disease. To address the relative paucity of information about their genomic status, molecular cytogenetic analysis was performed on the three recognized subtypes of canine melanocytic lesions. Using array comparative genomic hybridization (aCGH) analysis, highly aberrant distinct copy number status across the tumor genome for both of the malignant melanoma subtypes was revealed. The most frequent aberrations included gain of dog chromosome (CFA) 13 and 17 and loss of CFA 22. Melanocytomas possessed fewer genome wide aberrations, yet showed a recurrent gain of CFA 20q15.3-17. A distinctive copy number profile, evident only in oral melanomas, displayed a sigmoidal pattern of copy number loss followed immediately by a gain, around CFA 30q14. Moreover, when assessed by fluorescence in situ hybridization (FISH), copy number aberrations of targeted genes, such as gain of c-MYC (80% of cases) and loss of CDKN2A (68% of cases), were observed. This study suggests that in concordance with what is known for human melanomas, canine melanomas of the oral mucosa and cutaneous epithelium are discrete and initiated by different molecular pathways. Springer Science+Business Media Dordrecht, 2014 Canine nationallicence Oral melanoma nationallicence Cytogenetics nationallicence Array nationallicence Comparative genomic hybridization nationallicence BAC : Bacterial artificial chromosome nationallicence BRAF : V-raf murine sarcoma viral oncogene homolog B1 nationallicence C-KIT : V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog nationallicence C-MYC : V-myc myelocytomatosis viral oncogene homolog (avian) nationallicence CCND1 : G1/S-specific cyclin-D1 nationallicence CDK4 : Cyclin-dependent kinase 4 nationallicence CDKN1A : Cyclin-dependent kinase inhibitor 1A nationallicence CDKN2A : Cyclin-dependent kinase inhibitor 2A nationallicence CFA : Canis familiaris (also used as a prefix to chromosome numbers) nationallicence CNA : Copy number aberration nationallicence FFPE : Formalin-fixed paraffin-embedded nationallicence FISH : Fluorescence in situ hybridization nationallicence H&E : Hematoxylin and eosin nationallicence HSA : Homo sapiens (also used as a prefix to chromosome numbers) nationallicence MAP-K : Mitogen-activated protein kinases nationallicence oaCGH : Oligo-array comparative genomic hybridization nationallicence PTEN : Phosphatase and tensin homolog nationallicence RAS : Rat sarcoma gene nationallicence RB-1 : Retinoblastoma 1 nationallicence SLP : Single locus probe nationallicence SPRED1 : Sprouty-related, EVH1 domain containing 1 nationallicence TACC3 : Transforming, acidic coiled-coil containing protein 3 nationallicence TP53 : Cellular tumor antigen p53 nationallicence TRPM7 : Transient receptor potential cation channel, subfamily M, member 7 nationallicence Poorman Kelsey Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, 27607, Raleigh, NC, USA aut Borst Luke Department of Pathobiology and Population Health, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA aut Moroff Scott Antech Diagnostics, 1111 Marcus Avenue, Lake Success, NY, USA aut Roy Siddharth Department of Statistics, College of Physics and Applied Mathematics, North Carolina State University, Raleigh, NC, USA aut Labelle Philippe Antech Diagnostics, 1111 Marcus Avenue, Lake Success, NY, USA aut Motsinger-Reif Alison Department of Statistics, College of Physics and Applied Mathematics, North Carolina State University, Raleigh, NC, USA aut Breen Matthew Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, 27607, Raleigh, NC, USA aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 171-186 0967-3849 23:2<171 2015 23 10577 https://doi.org/10.1007/s10577-014-9444-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9444-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poorman Kelsey Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, 27607, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Borst Luke Department of Pathobiology and Population Health, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Moroff Scott Antech Diagnostics, 1111 Marcus Avenue, Lake Success, NY, USA aut NATIONALLICENCE 700 1- Roy Siddharth Department of Statistics, College of Physics and Applied Mathematics, North Carolina State University, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Labelle Philippe Antech Diagnostics, 1111 Marcus Avenue, Lake Success, NY, USA aut NATIONALLICENCE 700 1- Motsinger-Reif Alison Department of Statistics, College of Physics and Applied Mathematics, North Carolina State University, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Breen Matthew Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, 27607, Raleigh, NC, USA aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 171-186 0967-3849 23:2<171 2015 23 10577