caa a22 4500 606190783 CHVBK 20210128100857.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9446-4 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9446-4 Chromosome landmarks and autosome-sex chromosome translocations in Rumex hastatulus , a plant with XX/XY1Y2 sex chromosome system [Elektronische Daten] [Aleksandra Grabowska-Joachimiak, Adam Kula, Tomasz Książczyk, Joanna Chojnicka, Elwira Sliwinska, Andrzej Joachimiak] Rumex hastatulus is the North American endemic dioecious plant with heteromorphic sex chromosomes. It is differentiated into two chromosomal races: Texas (T) race characterised by a simple XX/XY sex chromosome system and North Carolina (NC) race with a polymorphic XX/XY1Y2 sex chromosome system. The gross karyotype morphology in NC race resembles the derived type, but chromosomal changes that occurred during its evolution are poorly understood. Our C-banding/DAPI and fluorescence in situ hybridization (FISH) experiments demonstrated that Y chromosomes of both races are enriched in DAPI-positive sequences and that the emergence of polymorphic sex chromosome system was accompanied by the break of ancestral Y chromosome and switch in the localization of 5S rDNA, from autosomes to sex chromosomes (X and Y2). Two contrasting domains were detected within North Carolina Y chromosomes: the older, highly heterochromatinised, inherited from the original Y chromosome and the younger, euchromatic, representing translocated autosomal material. The flow-cytometric DNA estimation showed ∼3.5% genome downsizing in the North Carolina race. Our results are in contradiction to earlier reports on the lack of heterochromatin within Y chromosomes of this species and enable unambiguous identification of autosomes involved in the autosome-heterosome translocation, providing useful chromosome landmarks for further studies on the karyotype and sex chromosome differentiation in this species. The Author(s), 2014 Rumex hastatulus nationallicence Sex chromosomes nationallicence Karyotype nationallicence FISH nationallicence rDNA nationallicence C-banding/DAPI nationallicence DAPI : 4′,6-diamidino-2-phenylindole nationallicence FISH : Fluorescence in situ hybridization nationallicence NC : North Carolina nationallicence PAR : Pseudoautosomal region nationallicence PCR : Polymerase chain reaction nationallicence T : Texas nationallicence Grabowska-Joachimiak Aleksandra Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Łobzowska 24, 31-140, Cracow, Poland aut Kula Adam Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Łobzowska 24, 31-140, Cracow, Poland aut Książczyk Tomasz Department of Environmental Stress Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland aut Chojnicka Joanna Department of Environmental Stress Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland aut Sliwinska Elwira Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789, Bydgoszcz, Poland aut Joachimiak Andrzej Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387, Cracow, Poland aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 187-197 0967-3849 23:2<187 2015 23 10577 https://doi.org/10.1007/s10577-014-9446-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9446-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grabowska-Joachimiak Aleksandra Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Łobzowska 24, 31-140, Cracow, Poland aut NATIONALLICENCE 700 1- Kula Adam Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Łobzowska 24, 31-140, Cracow, Poland aut NATIONALLICENCE 700 1- Książczyk Tomasz Department of Environmental Stress Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland aut NATIONALLICENCE 700 1- Chojnicka Joanna Department of Environmental Stress Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland aut NATIONALLICENCE 700 1- Sliwinska Elwira Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, 85-789, Bydgoszcz, Poland aut NATIONALLICENCE 700 1- Joachimiak Andrzej Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387, Cracow, Poland aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 187-197 0967-3849 23:2<187 2015 23 10577