caa a22 4500 606190791 CHVBK 20210128100857.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-014-9462-4 doi (NATIONALLICENCE)springer-10.1007/s10577-014-9462-4 New cryptic karyotypic differences between cattle ( Bos taurus ) and goat ( Capra hircus ) [Elektronische Daten] [Lisa De Lorenzi, Jordi Planas, Elena Rossi, Luca Malagutti, Pietro Parma] Cattle (Bos taurus) and goat (Capra hircus) belong to the Bovidae family, and they share a common ancestor 19.7-21.5Ma ago (MYA). The Bovidae family apparently experienced a rapid species radiation in the middle Miocene. The present day cattle and goat possess the same diploid chromosome number (2n = 60) and structurally similar autosomes, except that a small subcentromeric portion of cattle chromosome nine has been translocated to goat chromosome 14. In this study, we adopted a new strategy that involves the use of bioinformatics approach to detect unknown cryptic chromosome divergences between cattle and goat using and subsequent validation using the fluorescence in situ hybridization (FISH) of bacterial artificial chromosome clones. We identified two hypothetical discrepancies between the cattle and goat genome assemblies: an inversion in the goat chromosome 13 and a transposition in the goat chromosome 6. The FISH technique allowed clear validation of the existence of a new 7.4Mb chromosomal inversion in the goat chromosome 13. Regarding the transposition in the goat chromosome six, FISH analyses revealed that the cattle and goat genomes shared the same organization, with the assembly of the goat genome being the correct one. Moreover, we defined, for the first time, the size and orientation of the translocated fragment involved in the evolutionary translocation between cattle chromosomes 9 and goat chromosome 14. Our results suggest that bioinformatics represents an efficient method for detecting cryptic chromosome divergences among species. Springer Science+Business Media Dordrecht, 2015 Cattle nationallicence Goat nationallicence Chromosomes nationallicence Fish nationallicence MYA : Million years ago nationallicence FISH : Fluorescent in situ hybridization nationallicence CHI : Capra hircus nationallicence BTA : Bos taurus nationallicence Mb : Megabase nationallicence BAC : Bacteria artificial chromosome nationallicence INRA : Institut nationale de la recherche agronomique nationallicence Kb : Kilobase nationallicence CNV : Copy number variation nationallicence EBP : Evolutionary break point nationallicence De Lorenzi Lisa Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut Planas Jordi Department of System Biology, Vic University, Carrer de la Laura 13, 08500, Vic, Spain aut Rossi Elena Department of Molecular Medicine, Pavia University, Via Forlanini 14, 27100, Pavia, Italy aut Malagutti Luca Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut Parma Pietro Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 225-235 0967-3849 23:2<225 2015 23 10577 https://doi.org/10.1007/s10577-014-9462-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-014-9462-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Lorenzi Lisa Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Planas Jordi Department of System Biology, Vic University, Carrer de la Laura 13, 08500, Vic, Spain aut NATIONALLICENCE 700 1- Rossi Elena Department of Molecular Medicine, Pavia University, Via Forlanini 14, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Malagutti Luca Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Parma Pietro Department of Agricultural and Environmental Sciences, Milan University, Via Celoria 2, 20133, Milan, Italy aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 225-235 0967-3849 23:2<225 2015 23 10577